Inflammation Research

, Volume 51, Issue 7, pp 332–341

Genomic-scale analysis of gene expression profiles in TNF-α treated human umbilical vein endothelial cells

Authors

  • J. Zhou
    • The National Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China, Fax: ++86 10 62565689, e-mail: fengmf@panda.ioz.ac.cn
  • Y. Jin
    • Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
  • Y. Gao
    • Department of Cell Biology, College of Life Sciences, Peking University, Beijing 100871, China
  • H. Wang
    • The National Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China, Fax: ++86 10 62565689, e-mail: fengmf@panda.ioz.ac.cn
  • G. Hu
    • Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
  • Y. Huang
    • Department of Immunology, Beijing Anzhen Hospital, Beijing 100029, China
  • Q. Chen
    • The National Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China, Fax: ++86 10 62565689, e-mail: fengmf@panda.ioz.ac.cn
  • M. Feng
    • The National Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China, Fax: ++86 10 62565689, e-mail: fengmf@panda.ioz.ac.cn
  • C. Wu
    • The National Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China, Fax: ++86 10 62756876, e-mail: wuch@pku.edu.cn

DOI: 10.1007/PL00000312

Cite this article as:
Zhou, J., Jin, Y., Gao, Y. et al. Inflamm. res. (2002) 51: 332. doi:10.1007/PL00000312

Abstract.

Objective and design: TNF-α is a potent proinflammatory cytokine that plays an important role in immunity and inflammation, and in the control of cell proliferation, differentiation and programmed cell death. However, it is known that TNF-α is also the founding member of a still growing family of cytokines with diverse bioregulative functions. Its detailed molecular mechanisms on endothelial activation and injury remain to be elucidated. This study was aimed at determining genomic-scale gene expression profiles in TNF-α treated human endothelial cells.¶Materials and methods: In this study cultured human umbilical vein endothelial cells (HUVECs) were stimulated with TNF-α (10 ng/ml) for 2 and 16 h, respectively, and the gene expression pattern was profiled using a cDNA array representing 14000 gene/cDNA clusters.¶Results: In total, 72 known human genes were identified the expression levels of which altered over 2-fold in response to TNF-α stimulation. Such alteration was confirmed for IL-8 and MCP-1, with an independent quantitative mRNA assay. It was observed that genes with related biological functions were often temporally co-regulated.¶Conclusions: These results indicate the transcriptional pathways mediated by TNF-α inside the HUVECs. Expression profiling in HUVECs responding to TNF-α stimulation should give an understanding of the molecular mechanisms involved in vascular inflammation.

Key words: TNF-α - HUVECs - cDNA array - Gene expression profile

Copyright information

© Birkhäuser Verlag, 2002