Inflammation Research

, Volume 50, Issue 9, pp 449–452

Localisation of mRNAs for diamine oxidase and histamine receptors H1 and H2, at the feto-maternal interface of human pregnancy

  • O.B. Brew
  • M.H.F. Sullivan

DOI: 10.1007/PL00000269

Cite this article as:
Brew, O. & Sullivan, M. Inflamm. res. (2001) 50: 449. doi:10.1007/PL00000269

Abstract.

Objective and design: To localise mRNAs for the histamine receptors H1, H2 and H3, and for diamine oxidase, in the placenta and decidua of the human feto-maternal interface.¶Materials and methods: Complementary DNA for each mRNA of interest was amplified by polymerase chain reaction. Sub-cloned sequences were used to prepare probes for in situ hybridisation, and these were employed to localise the expression of mRNAs for histamine receptors H1 and H2, and for diamine oxidase.¶Results: mRNA for histamine receptors H1 and H2, and for diamine oxidase could be detected at the feto-maternal interface of human pregnancy, and localised to both decidual and placental cells.¶Conclusion: The co-expression of these receptors and DAO is consistent with a role for histamine at the feto-maternal interface of human pregnancy.

Key words: Histamine receptors H1, H2 - Diamine oxidase - Placenta - Decidua

Copyright information

© Birkhäuser Verlag, 2001

Authors and Affiliations

  • O.B. Brew
    • 1
  • M.H.F. Sullivan
    • 2
  1. 1.Wolfson Institute of Health Science, Thames Valley University, 32-38 Uxbridge Road, London W5 2BS, UK, Fax: ++44 208 231 2442, e-mail: obed.brew@tvu.ac.nkGB
  2. 2.Institute of Reproductive & Developmental Biology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UKGB