Abstract
In genetically predisposed individuals, autoimmune lymphocytic thyroiditis (LT) is potentiated by excess dietary iodine (I). There have been data which suggest that oxidative stress may have a role in iodine-induced LT. These in vivo studies were undertaken to examine the effect of iodine on intrathyroidal levels of the potent antioxidant glutathione (GSH) and see if the thyroids of LT-prone BB/Wor rats have aberrant GSH responses after iodine-loading. LT-prone BB/Wor, non LT-prone BB/Wor and Wistar rats were randomized to receive either 0.05% I (as Nal) or tap water. Thyroid and liver homogenates were assayed individually for GSH. Following the administration of 0.05% iodine water overnight, all of the animals demonstrated a rise in intrathyroidal GSH regardless of LT-proneness. To determine whether this was a dose-dependent response, Wis rats were randomized to receive tap, 0.0125%, 0.025%, 0.05%, or 0.075% I, overnight. Intrathyroidal GSH levels rose with increasing iodine concentrations peaking at 0.025% I. Hepatic GSH levels were unaltered by iodine treatment. Ten days of 0.05% I water did not result in any difference between the GSH levels of thyroids from treated and control rats. Frozen sections of the thyroids and livers from iodine-treated rats were compared to tap-water controls after staining with Mercury Orange for GSH and Schiffs reagent for evidence of lipid peroxidation. Iodine-treated thyroids had an apparent shift of GSH staining from the apical border to the cytoplasm. However, there was no Schiffs staining indicative of lipid peroxidation in the iodine-treated thyroids. These results show that acute iodine loads increase intrathyroidal GSH and LT-prone rats do not have an inherent deficit in this response. Therefore, we conclude that there is no role for oxidative damage in iodine-induced LT. We propose that GSH has a role in intrathyroidal iodine metabolism and thyroid hormone synthesis.
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References
Wolff J. Iodide goiter and the pharmacologic effects of excess iodide. Am. J. Med. 47: 101, 1969.
Wolff J. Excess iodide inhibits the thyroid by multiple mechanisms. Adv. Exp. Med. Biol. 267: 211, 1989.
Sundick R.S., Bagchi N., Brown T.R. The role of iodine in thyroid autoimmunity — from chickens to humans. Autoimmunity 13: 61, 1992.
Beierwaltes W.H. Iodide and lymphocytic thyroiditis. Bull. All. India. Inst. Med. Sci. 3: 145, 1969.
Braverman L.E., Ingbar S.H., Vagenakis A.G., Adams L, Maloof F. Enhanced susceptibility to iodine myxedema in patients with Hashimoto’s disease. J. Clin. Endocrinol. Metab. 32: 515, 1971.
Tajiri J., Higashi K., Morita M., Umeda T., Sato T. Studies of hypothyroidism in patients with high iodine intake. J. Clin. Endocrinol. Metab. 63: 412, 1986.
Harach H.R., Escalante D.A., Onativia A., Outes J.L., Day E.S., Williams E.D. Thyroid carcinoma and thyroiditis in an endemic goitre region before and after iodine prophylaxis. Acta Endocrinol. (Copenh.) 108: 55, 1985.
Sternthal E., Like A.A., Sarantis K., Braverman L.E. Lymphocytic thyroiditis and diabetes in the BB/W rat. Diabetes 30: 1058, 1981.
Rajatanavin R., Appel M.C., Reinhardt W., Alex S., Yang Y., Braverman L.E. Variable prevalence of lymphocytic thyroiditis among diabetes-prone sublines of BB/Wor rats. Endocrinology 128: 153, 1991.
Allen E.M., Appel M.C., Braverman L.E. The effect of iodine ingestion on the development of spontaneous lymphocytic thyroiditis in the diabetes-prone BB/W rat. Endocrinology 178: 1977, 1986.
Alex S., Norvell B., Ebner S. Effect of excess iodine on the incidence of lymphocytic thyroiditis in the BB/Wor rat-a time course study. Sixty-sixth ATA Meeting, Rochester MN, 1992, S–75 (Abstract).
Bagchi N., Brown T.R., Urdaniva E., Sundick R.S. Induction of autoimmune thyroiditis in chickens by dietary iodine. Science 230: 325, 1985.
Evans T.C., Beierwaltes W.H., Nishiyama R.H. Experimental canine Hashimoto’s thyroiditis. Endocrinology 84: 641, 1969.
Allen E.M., Braverman L.E. The effect of iodine on lymphocytic thyroiditis in the thymectomized Buffalo rat. Endocrinology 127: 1613, 1990.
Sundick R.S., Herdegen D.M., Brown T.R., Bagchi N. The incorporation of dietary iodine into thyroglobulin increases its antigenicity. Endocrinology 120: 2078, 1987.
Champion B.R., Rayner D.C., Byfield P.G., Page K.R., Chan CT., Roitt I.M. Critical role of iodination for T cell recognition of thyroid autoimmunity. J. Immunol. 739: 3665, 1987.
Bagchi N., Brown T.R., Herdegen D.M., Dhar A., Sundick R.S. Antioxidants delay the onset of thyroiditis in Obese Strain chickens. Endocrinology 127: 1590, 1990.
Many M.C., Mestdagh C., Van Den Hove M.F., Denef J.F. In vitro study of acute toxic effects of high iodide doses in human thyroid follicles. Endocrinology 131: 621, 1992.
Allen E.M. The effect of iodine on lipid peroxidation and ultra-structure in the thyroids of BB/WOR rats. J. Endocrinol. Invest. 15: 159, 1992.
Deneke S.M., Fanburg B.L. Regulation of cellular glutathione. Am. J. Physiol. 257: 163, 1989.
Shan X., Aw T.Y., Jones D.P. Glutathione-dependent protection against oxidative injury. Pharmacol. Ther. 47: 61, 1990.
Sedlak J., Lindsay R.H. Estimation of total, protein-bound, and nonprotein-bound sulfhydryl groups in tissue with Ellman’s reagen Anal. Biochem. 25: 192, 1968.
Mokrasch L.C., Teschke E.J. Glutathione content of cultured cells and rodent brain regions: a specific fluorometric assay. Anal. Biochem. 140: 506, 1984.
Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265, 1951.
Forkert P.G., Moussa M. Histologic localization of glutathione in fixed tissues Histochem. J. 21: 634, 1989.
Pompella A., Maellaro E., Casini A.F., Comporti M. Histochemical detection of lipid peroxidation in the liver of bromobenzene-poisoned mice. Am. J. Pathol. 129: 295, 1987.
Follis R.H. Thyroiditis resulting from administration of excess iodine to hamsters with hyperplastic goiters. Proc. Soc. Exp. Biol. Med. 102: 425, 1959.
Follis R.H. Further observations on thyroiditis and colloid accumulation in hyperplastic thyroid glands of hamsters receiving excess iodine. Lab. Invest. 13: 1590, 1964.
Krupp P.P., Lee K.P. The effects of dietary iodine on thyroid ultrastructure. Tissue Cell 20: 79, 1988.
Mahmoud J., Colin J., Many M.C., Denef J.F. Direct toxic effects of iodine in excess on iodine-deficient thyroid glands. Exp. Mol. Pathol. 44: 259, 1986.
Sedlak J. Long-term effects of hypophysectomy on various fractions of sulfhydryl groups in thyroid, adrenal, and some other organs in the rat. Endocrinol. Exp. 19: 186, 1985.
Wolff J., Chaikoff I.L. Plasma inorganic iodide as a homeostatic regulator of thyroid function. J. Biol. Chem. 174: 555, 1948.
Schussler G.C., Ingbar S.H. The role of intermediary carbohydrate metabolism in regulating organic iodinations in the thyroid gland. J. Clin. Invest. 40: 1394, 1961.
Nakamura M., Yamazaki I., Kotani T., Ohtaki S. Thyroglobulin-mediated one- and two-electron oxidations of glutathione and ascorbate in thyroid peroxidase systems. J. Biol. Chem. 264: 12909, 1989.
Ekholm R. Biosynthesis of thyroid hormones. Int. Rev. Cytol. 120: 243, 1990.
Hwang C, Sinskey A.J., Lodish H.F. Oxidized redox state of glutathione in the endoplasmic reticulum. Science 257: 1496, 1992.
Kim P.S., Arvan P. Folding and assembly of newly synthesized thyroglobulin occurs in a pre-golgi compartment. J. Biol. Chem. 266: 12412, 1991.
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Supported by the Veterans Administration Merit Review, U.S.A.
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Allen, E.M. Acute iodine ingestion increases intrathyroidal glutathione. J Endocrinol Invest 16, 265–270 (1993). https://doi.org/10.1007/BF03348827
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DOI: https://doi.org/10.1007/BF03348827