Abstract
Suspension cultures of Indics (Basmati 370 and Improved Sabarmati) and Japonica (Taipei 309) rice were established from scutellum-derived callus obtained by culture of the mature seeds. The suspension cultures contain groups of small and cytoplasmically rich cells with a Qubling time of 3.6–5.1 days, as measured by Increase In packed cell volume. The suspension cells of Indica rice var Improved Sabarmati were employed to optimize electroporation-mediated delivery of the plasmid pBl 221 having β-glucuronidase gene under the control of eukaryotic expression signals. GUS activity was determined both fluorometrically and hisotchemlcally. Maximum expression was obtained when cells were electroporated at 600 V cm-1 with capacitance selected at 400 μF.
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References
Kyozuka J & Shimamoto K, In Transgenic plants: Fundamentals and applications (A Hiatt, Editor), MarcelDekker, Inc, New York (1993)193.
Wang V-C, Klein TM, Fromm, M, Cao J, Sanford JC & Wu R, Plant Mol Biol, 11 (1989) 433.
Bruce WB, Christensen AH, Klein T, Fromm M & Quail PH, Proc Natl Acad Sci, USA 86 (1989) 9692.
Oard JH, Paige OF, Simmonds JA & Gradziel TM, Plant Physiol, 92 (1990) 334.
Christou P, Ford TL & Kofron M, Biol Technology, 9 (1991) 957.
Cao J, Duan X, McElroy D & Wu R, Plant Cell Rep, 11 (1992) 586.
Li L, Qu R, de Kochko A, Fauquet C & Beachy RN, Plant Cell Rep, 12 (1993) 250.
Lee N, Wang Y, Yang J, Ge K, Huang S & Tan J, Proc Natl Acad Sci, USA, 88 (1991) 6389.
Topfer R, Gronenborn B, Schell J & Steinbiss H-H, Plant Cell, 1 (1999) 133.
Dekeyser RA, Claes B, De Rycke RMU, Habets ME, Van Montagu MC & Caplan AB, Plant Cell, 2 (1990) 591.
O’Hailuln K, Bonne E, Bossut M, De Beuckeleer M & Leemans J, Plant Cell, 4 (1992) 1495
Songstad DO, Halaka FG, DeBoer DL, Armstrong CL, Hinchee MAW, Ford-Salino CG, Brown SM, Fromm ME & Horsch RB, Plant Cell, Tissue & Org Cult 33 (1993) 195.
Kloti A, Iglesias VA, Wilnn J, Burkhardt PK, Datta SK & Potrykus I, Plant Cell Rep, 12 (1993) 671.
Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY & Bi FY, Sci Sinica, 18 (1975) 659.
Muller AJ & Grate R, Mol Gen Genet, 161 (1978) 67.
Jefferson RA, Kavanagh TA & Bevan MW, EMBO J, 6 (1987) 3901.
Bimbolm HC & Doly J, Nucleic Acids Res, 7 (1979) 1513.
Chaudhury A, Maheshwarl SC & Tyagl AK, Physiol Plant, 89 (1993) 842.
Vasil IK, Biol Technology, 6 (1988) 397.
Torlyama K & Hinata K, Plant Sci, 41 (1985) 179.
Ghosh Biswas GC & Zapata FJ, J Plant Physiol, 139 (1992) 523.
Manoharan K & Gnanam A, Plant Cell Physiol, 33 (1992) 1243.
Horsch RB & Jones GE, In Vitro, 16 (1980) 103.
Lindsey K & Jones MGK, Plant Mol Biol, 10 (1987) 43.
Dekeyser R, Claea B, Marichal M, Van Montagu M & Caplan A, Plant Physiol, 90 (1989) 217.
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Chaudhury, A., Chowdhry, C.N., Maheshwar, N. et al. Growth Behaviour of Suspension Cultures of Rice and Transient Expression of Electroporated Gene in Intact Cells. J. Plant Biochem. Biotechnol. 3, 9–13 (1994). https://doi.org/10.1007/BF03321941
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DOI: https://doi.org/10.1007/BF03321941