Skip to main content
Log in

Growth Behaviour of Suspension Cultures of Rice and Transient Expression of Electroporated Gene in Intact Cells

  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Suspension cultures of Indics (Basmati 370 and Improved Sabarmati) and Japonica (Taipei 309) rice were established from scutellum-derived callus obtained by culture of the mature seeds. The suspension cultures contain groups of small and cytoplasmically rich cells with a Qubling time of 3.6–5.1 days, as measured by Increase In packed cell volume. The suspension cells of Indica rice var Improved Sabarmati were employed to optimize electroporation-mediated delivery of the plasmid pBl 221 having β-glucuronidase gene under the control of eukaryotic expression signals. GUS activity was determined both fluorometrically and hisotchemlcally. Maximum expression was obtained when cells were electroporated at 600 V cm-1 with capacitance selected at 400 μF.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kyozuka J & Shimamoto K, In Transgenic plants: Fundamentals and applications (A Hiatt, Editor), MarcelDekker, Inc, New York (1993)193.

    Google Scholar 

  2. Wang V-C, Klein TM, Fromm, M, Cao J, Sanford JC & Wu R, Plant Mol Biol, 11 (1989) 433.

    Article  CAS  Google Scholar 

  3. Bruce WB, Christensen AH, Klein T, Fromm M & Quail PH, Proc Natl Acad Sci, USA 86 (1989) 9692.

    Article  PubMed  CAS  Google Scholar 

  4. Oard JH, Paige OF, Simmonds JA & Gradziel TM, Plant Physiol, 92 (1990) 334.

    Article  PubMed  CAS  Google Scholar 

  5. Christou P, Ford TL & Kofron M, Biol Technology, 9 (1991) 957.

    Article  Google Scholar 

  6. Cao J, Duan X, McElroy D & Wu R, Plant Cell Rep, 11 (1992) 586.

    Article  CAS  Google Scholar 

  7. Li L, Qu R, de Kochko A, Fauquet C & Beachy RN, Plant Cell Rep, 12 (1993) 250.

    Article  Google Scholar 

  8. Lee N, Wang Y, Yang J, Ge K, Huang S & Tan J, Proc Natl Acad Sci, USA, 88 (1991) 6389.

    Article  PubMed  CAS  Google Scholar 

  9. Topfer R, Gronenborn B, Schell J & Steinbiss H-H, Plant Cell, 1 (1999) 133.

    Google Scholar 

  10. Dekeyser RA, Claes B, De Rycke RMU, Habets ME, Van Montagu MC & Caplan AB, Plant Cell, 2 (1990) 591.

    PubMed  CAS  Google Scholar 

  11. O’Hailuln K, Bonne E, Bossut M, De Beuckeleer M & Leemans J, Plant Cell, 4 (1992) 1495

    Google Scholar 

  12. Songstad DO, Halaka FG, DeBoer DL, Armstrong CL, Hinchee MAW, Ford-Salino CG, Brown SM, Fromm ME & Horsch RB, Plant Cell, Tissue & Org Cult 33 (1993) 195.

    Article  CAS  Google Scholar 

  13. Kloti A, Iglesias VA, Wilnn J, Burkhardt PK, Datta SK & Potrykus I, Plant Cell Rep, 12 (1993) 671.

    Article  Google Scholar 

  14. Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.

    Article  CAS  Google Scholar 

  15. Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY & Bi FY, Sci Sinica, 18 (1975) 659.

    Google Scholar 

  16. Muller AJ & Grate R, Mol Gen Genet, 161 (1978) 67.

    Article  Google Scholar 

  17. Jefferson RA, Kavanagh TA & Bevan MW, EMBO J, 6 (1987) 3901.

    PubMed  CAS  Google Scholar 

  18. Bimbolm HC & Doly J, Nucleic Acids Res, 7 (1979) 1513.

    Article  Google Scholar 

  19. Chaudhury A, Maheshwarl SC & Tyagl AK, Physiol Plant, 89 (1993) 842.

    Article  CAS  Google Scholar 

  20. Vasil IK, Biol Technology, 6 (1988) 397.

    Article  Google Scholar 

  21. Torlyama K & Hinata K, Plant Sci, 41 (1985) 179.

    Article  Google Scholar 

  22. Ghosh Biswas GC & Zapata FJ, J Plant Physiol, 139 (1992) 523.

    Article  Google Scholar 

  23. Manoharan K & Gnanam A, Plant Cell Physiol, 33 (1992) 1243.

    CAS  Google Scholar 

  24. Horsch RB & Jones GE, In Vitro, 16 (1980) 103.

    Article  Google Scholar 

  25. Lindsey K & Jones MGK, Plant Mol Biol, 10 (1987) 43.

    Article  CAS  Google Scholar 

  26. Dekeyser R, Claea B, Marichal M, Van Montagu M & Caplan A, Plant Physiol, 90 (1989) 217.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chaudhury, A., Chowdhry, C.N., Maheshwar, N. et al. Growth Behaviour of Suspension Cultures of Rice and Transient Expression of Electroporated Gene in Intact Cells. J. Plant Biochem. Biotechnol. 3, 9–13 (1994). https://doi.org/10.1007/BF03321941

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03321941

Key words

Navigation