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An efficient In Vitro Method for Mass Propagation of Salvadora persica via Apical Meristem

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Abstract

A rapid clonal propagation system has been developed for medicinally potent tree species - Salvadora persica via single step through apical meristem. Shoot tips (1 cm) were inoculated on MS medium incorporated with 17.8 µM benzyladenine and 18.6 µM kinetin along with activated charcoal (0.3%).The shoot tips grew fast (4 cm) within 7 days alongwith well developed roots on the same medium.Thus, the present investigation demonstrates the complete regeneration of Salvadora persicaplants via single step. Such reproducible protocol could be used for the production of virus-free plantlets in quicker time.

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Abbreviations

BA:

bemyladenine

Kn:

kinetin

MS:

Murashige & Skoog’s

SD:

Standard deviation

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Authors and Affiliations

  1. Biotechnology Laboratory, Department of Botany, University of Rajasthan, Jaipur, 302 004, India

    Sujata Mathur, Gyan Singh Shekhawat & Amla Batra

Authors
  1. Sujata Mathur
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  2. Gyan Singh Shekhawat
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  3. Amla Batra
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Mathur, S., Singh Shekhawat, G. & Batra, A. An efficient In Vitro Method for Mass Propagation of Salvadora persica via Apical Meristem. J. Plant Biochem. Biotechnol. 11, 125–127 (2002). https://doi.org/10.1007/BF03263149

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  • DOI: https://doi.org/10.1007/BF03263149

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