Molecular Diagnosis & Therapy

, Volume 10, Issue 6, pp 397–403

Detection of α-Methylacyl-Coenzyme-A Racemase Transcripts in Blood and Urine Samples of Prostate Cancer Patients

  • Barbara  K. Zehentner
  • Heather Secrist
  • XinQun Zhang
  • Dawn C. Hayes
  • Richard Ostenson
  • Gary Goodman
  • Jiangchun Xu
  • Mark Kiviat
  • Nancy Kiviat
  • David H. Persing
  • Raymond L. Houghton
Original Research Article

DOI: 10.1007/BF03256217

Cite this article as:
Zehentner, B. ., Secrist, H., Zhang, X. et al. Mol Diag Ther (2006) 10: 397. doi:10.1007/BF03256217

Abstract

Background: α-Methylacyl-coenzyme-A racemase (AMACR) has been shown to be a highly specific marker for prostate cancer cells, even in the earliest stages of malignant progression. It is expressed at much higher levels than prostate-specific antigen (PSA) in malignant tissues, and is not expressed at appreciable levels in normal prostatic epithelium. In this study, we demonstrate the quantitative detection of AMACR transcripts in peripheral blood of prostate cancer patients using real-time RT-PCR. In addition, we have undertaken a pilot study to demonstrate the potential application of this technique for the detection of prostate tumor cells in urine samples from patients with prostate cancer.

Methods: A real-time RT-PCR assay was developed for detection of the expression of AMACR in prostate cancer patients. Blood samples from 163 patients were tested at various stages of disease progression, with or without therapy. Blood specimens from patients with benign prostate disorders and other types of cancer were also evaluated.

Results: In 28 of 58 samples from patients with known metastatic disease who were undergoing treatment, an AMACR expression signal above the cut-off value was detected, consistent with the presence of circulating tumor cells. In 39 of 88 patients with presumptive organ-confined disease, there was evidence of low levels of circulating tumor cells. Comparison of AMACR RT-PCR with known serum PSA values indicated that a combination of these parameters significantly increased the sensitivity for detection of progressive disease. In a pilot study analyzing urine samples from seven prostate cancer patients, elevated AMACR expression levels were detected in the urine sediments of four of six stage-T1 prostate cancer patients and in the one patient with stage-T2 prostate cancer.

Conclusion: The data presented in this study indicates that AMACR real-time RT-PCR may aid in the detection and staging of prostate cancer.

Copyright information

© Adis Data Information BV 2006

Authors and Affiliations

  • Barbara  K. Zehentner
    • 1
  • Heather Secrist
    • 2
  • XinQun Zhang
    • 2
  • Dawn C. Hayes
    • 2
  • Richard Ostenson
    • 3
  • Gary Goodman
    • 4
  • Jiangchun Xu
    • 5
  • Mark Kiviat
    • 6
  • Nancy Kiviat
    • 6
  • David H. Persing
    • 7
  • Raymond L. Houghton
    • 8
  1. 1.Hematologics Inc.SeattleUSA
  2. 2.GSK-BioSeattleUSA
  3. 3.Good Samaritan HospitalPuyallupUSA
  4. 4.Swedish HospitalSeattleUSA
  5. 5.Amgen Inc.SeattleUSA
  6. 6.Harborview Medical CenterSeattleUSA
  7. 7.CepheidSunnyvaleUSA
  8. 8.Inbios International Inc.SeattleUSA