Journal of Applied Genetics

, Volume 47, Issue 4, pp 309–318

Agrobacterium-mediated transformation of yellow lupin to generate callus tissue producing HBV surface antigen in a long-term culture

  • Tomasz Pniewski
  • Józef Kapusta
  • Andrzej Płucienniczak
Original Article

DOI: 10.1007/BF03194640

Cite this article as:
Pniewski, T., Kapusta, J. & Płucienniczak, A. J Appl Genet (2006) 47: 309. doi:10.1007/BF03194640

Abstract

The idea of an oral vaccine administered as a portion of plant tissue requires a high level of antigen production. An improved protocol for the induction of transgenic yellow lupin calli or tumours, reaching 44% of transformation rate, is presented here. It has been developed by using thenptII marker gene and theuidA reporter gene as well as variousAgrobacterium strains and plant explants. This method of seedling and hypocotyl transformation was applied to raise calli or tumours producing a small surface antigen of Hepatitis B Virus (S-HBsAg). Lupin tissue lines were long-term cultured on selection media maintaining the growth rate and high expression level of the native form of S-HBs, up to 6 μg per g of fresh tissue.

Key words

Agrobacterium tumefacienscallusgenetic transformationHBsAgHepatitis B VirusLupinus luteussurface antigen

Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2006

Authors and Affiliations

  • Tomasz Pniewski
    • 1
  • Józef Kapusta
    • 1
    • 2
  • Andrzej Płucienniczak
    • 2
  1. 1.Institute of Plant GeneticsPolish Academy of SciencesPoznańPoland
  2. 2.Institute of Biotechnology and AntibioticsWarszawaPoland