Science in China Series C: Life Sciences

, Volume 42, Issue 3, pp 323–329

cDNA cloning and expression of an apoptosis-related gene, humanTFAR15 gene

  • Yugang Wang
  • Hongtao Liu
  • Yingmei Zhang
  • Dalong Ma
Article

DOI: 10.1007/BF03183610

Cite this article as:
Wang, Y., Liu, H., Zhang, Y. et al. Sci. China Ser. C.-Life Sci. (1999) 42: 323. doi:10.1007/BF03183610

Abstract

By means of cDNA-RDA method, some cDNA fragments were found to have high levels of expression during deprivation of GM-CSF (granulocyte macrophage-colony stimulating factor) in a human myeloid cell line, TF-1 cells. One of these fragments was identified as a novel gene. To get the full length of cDNA, rapid amplification of cDNA ends (RACE) and expressed sequence tags (EST) overlapping fragments assembling strategies were used. The novel gene was named TRAF15 (TF-1 cell apoptosis related gene-15), which consists of 1 218 nucleotides and encodes 212 amino acids. The putative protein product of TFAR15 is partially homologous toC. elegans protein C14A4.11. TFAR15 mRNA is expressed in fetal liver, kidney, spleen and lung, and also in some human myeloid cell lines. Both of the TFAR15 mRNA and protein were highly expressed in TF-1 cells after GM-CSF withdrawal.In vitro analysis showed that the recombinant TFAR15 protein could inhibit the natural cell death of 293 cells, an embryonic kidney cell line.

Keywords

apoptosiscDNA-RDARACETF-1 cell line293 cells

Copyright information

© Science in China Press 1999

Authors and Affiliations

  • Yugang Wang
    • 1
  • Hongtao Liu
    • 1
  • Yingmei Zhang
    • 1
  • Dalong Ma
    • 1
  1. 1.Laboratory of Medical ImmunologyBeijing Medical UniversityBeijingChina