Chinese Science Bulletin

, Volume 49, Issue 17, pp 1806–1811

Development of the CAS-LIBB single-particle microbeam for localized irradiation of living cells

Authors

  • Xufei Wang
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Xiaohua Wang
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Lianyun Chen
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Zhiwen Hu
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Jun Li
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Yu Wu
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Bin Chen
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Suhua Hu
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Jun Zhang
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Mingliang Xu
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Lijun Wu
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Shaohu Wang
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Huiyun Feng
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Furu Zhan
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Shixiang Peng
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Chundong Hu
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Shuqing Zhang
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Jianjun Cheng
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Zhongtao Shi
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Hang Yuan
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
  • Haitao Yuan
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering, Institute of Plasma PhysicsChinese Academy of Sciences
    • Key Laboratory of Ion Beam Bioengineering in Anhui ProvinceChinese Academy of Sciences
Articles

DOI: 10.1007/BF03183404

Cite this article as:
Wang, X., Wang, X., Chen, L. et al. Chin.Sci.Bull. (2004) 49: 1806. doi:10.1007/BF03183404

Abstract

A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydrogen ions, produced by a van de Graaff accelerator, in an energy range of 2.0–3.0 MeV, into an area smaller than that of the nucleus of an individual living cell. The beam is collimated by a borosilicate glass capillary that forms the beam-line exit. An integrated computer program recognizes the cells and locates them one by one over the microbeam exit for irradiation. We present technical details of the CAS-LIBB microbeam facility, particularly on the collimator, hardware, control program, as well as cell irradiation protocols available. Various factors contributing to the targeting and positioning precision are discussed along with accuracy measurement results.

Keywords

microbeam facilitysingle-particle irradiationparticle detection precisiontarget location accuracyliving cells

Copyright information

© Science in China Press 2004