Archives of Pharmacal Research

, Volume 26, Issue 1, pp 58–63

Ginsenoside-Rb1 acts as a weak phytoestrogen in MCF-7 human breast cancer cells

  • Young Joo Lee
  • Young Ran Jin
  • Won Chung Lim
  • Wan Kyu Park
  • Jung Yoon Cho
  • Siyoul Jang
  • Seung Ki Lee
Research Articles Articles

DOI: 10.1007/BF03179933

Cite this article as:
Lee, Y.J., Jin, Y.R., Lim, W.C. et al. Arch Pharm Res (2003) 26: 58. doi:10.1007/BF03179933

Abstract

Ginseng has been recommended to alleviate the menopausal symptoms, which indicates that components of ginseng very likely contain estrogenic activity. We have examined the possibility that a component ofPanax ginseng, ginsenoside-Rb1, acts by binding to estrogen receptor. We have investigated the estrogenic activity of ginsenoside-Rb1, in a transient transfection system using estrogen-responsive luciferase plasmids in MCF-7 cells. Ginsenoside-Rbb1 activated the transcription of the estrogen-responsive luciferase reporter gene in MCF-7 breast cancer cells at a concentration of 50 μM. Activation was inhibited by the specific estrogen receptor antagonist ICI 182,780, indicating that the estrogenic effect of ginsenoside-Rbb1 is estrogen receptor dependent. Next, we evaluated the ability of ginsenoside-Rbb1, to induce the estrogen-responsive gene c-fos by semi-quantitative RT-PCR assays and Western analyses. Ginsenoside-Rbb1 increased c-fos both at mRNA and protein levels. However, ginsenoside-Rbb1 failed to activate the glucocorticoid receptor, the retinoic acid receptor, or the androgen receptor in CV-1 cells transiently transfected with the corresponding steroid hormone receptors and hormone responsive reporter plasmids. These data support our hypothesis that ginsenoside-Rbb1 acts a weak phytoestrogen, presumably by binding and activating the estrogen receptor.

Key words

GinsengGinsenoside-Rbb1Estrogen receptorPhytoestrogen

Copyright information

© The Pharmaceutical Society of Korea 2003

Authors and Affiliations

  • Young Joo Lee
    • 1
  • Young Ran Jin
    • 2
  • Won Chung Lim
    • 1
  • Wan Kyu Park
    • 1
  • Jung Yoon Cho
    • 1
  • Siyoul Jang
    • 3
  • Seung Ki Lee
    • 2
  1. 1.College of Engineering, Institute of Biotechnology, Department of Bioscience and BiotechnologySejong UniversitySeoulKorea
  2. 2.College of PharmacySeoul National UniversitySeoulKorea
  3. 3.College of EngineeringKookmin UniversitySeoulKorea