Proceedings of the Indian Academy of Sciences - Section B

, Volume 58, Issue 3, pp 128-136

First online:

The nucleus ofSaccharomyces bayanus

  • N. V. AswathanarayanaAffiliated withCytogenetics Laboratory, Department of Biochemistry, Indian Institute of Science

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The extra-vacuolar nucleus is visible in a small percentage of living cells from 72–96 hour wort cultures. The vacuoles show a luminous boundary under dark ground illumination. The details observed in living nuclei could be stained with haematoxylin after fixation in iodine-formaldehyde-acetic acid mixture. The Feulgen-negative nature of the vacuole and the limitation of the Feulgen-positive material to the area bounded by the nuclear membrane would imply that the ‘centrosome’ described by Lindegren and Rafalko (1950) is the real nucleus. The nucleus ofS. bayanus conforms in its structure to those of higher organisms.