Pathology & Oncology Research

, Volume 7, Issue 4, pp 292–297

New diagnostic tool for differentiation of idiopathic hypereosinophilic syndrome (HES) and secondary eosinophilic states

  • Tímea Berki
  • Marianna Dávid
  • Beáta Bóné
  • Hajna Losonczy
  • János Vass
  • Péter Németh
Article

DOI: 10.1007/BF03032386

Cite this article as:
Berki, T., Dávid, M., Bóné, B. et al. Pathol. Oncol. Res. (2001) 7: 292. doi:10.1007/BF03032386

Abstract

The hypereosinophilic syndrome (HES) is a very rare disease, characterized by persistent eosinophilia with tissue involvement and organ dysfunction which often precedes a subsequent T cell lymphoma. Interleukin-5 secreted by a T lymphocyte subpopulation has been described in previous reports as the most important factor responsible for the prolonged lifespan of the eosinophils. The goal of the present study was to describe a fast, simple diagnostic method for the differentiation of HES and secondary eosinophilic states. Beside the surface marker analysis of peripheral blood mononu-clear cells (PBMC) we measured surface bound IgE molecules on lymphocytes and eosinophil cells, intracellular cytokines (IL-5, INFγ) in CD4+ lymphocytes and eosinophil major basic protein (MBP) in eosinophils using flow cytometric detection method. The appearance of an IL-5 producing cell population with a decreased number of INFγ positive lymphocytes was characteristic for the blood samples of HES patients. Predominance of Th2 cells with the appearance of a CD8+/CD3-/CD56+ cell population was restricted for the HES cases and could not be detected in secondary eosinophilic individuals. Our flow cytometric cytokine detection method (with parallel cell surface marker analysis) does not require cell separation or long term cell culture steps previously described for the detection of IL-5 producing cells. Therefore it seems to be a more appropriate approach for the differential diagnosis of primary and secondary eosinophilic states.

Keywords

hypereosinophilic syndromeIL-5INFγTh1-Th2 balanceflow cytometry

Copyright information

© Arányi Lajos Foundation 2001

Authors and Affiliations

  • Tímea Berki
    • 1
  • Marianna Dávid
    • 2
  • Beáta Bóné
    • 1
  • Hajna Losonczy
    • 2
  • János Vass
    • 3
  • Péter Németh
    • 1
  1. 1.Department of Immunology and BiotechnologyUniversity of Pécs, Medical FacultyPécsHungary
  2. 2.1st Department of Internal MedicineUniversity of Pécs, Medical FacultyHungary
  3. 3.Department of PathologyUniversity of Pécs, Medical FacultyHungary