Journal of Plant Biology

, Volume 51, Issue 1, pp 48–51

TohpreproHypSys- A Gene Expression and Defense Protein Activity in the Tobacco Wounding Response


DOI: 10.1007/BF03030740

Cite this article as:
Ren, F., Lian, Hj. & Chen, L. J. Plant Biol. (2008) 51: 48. doi:10.1007/BF03030740


Tobacco hydroxyproline-rich glycopeptide systemin precursor A (TobpreproHypSys-A), from which TobHypSys I and II are released, plays a crucial role in defense responses. Here, we investigated the expression otTobpreproHypSys- A and the activity of defense proteins in tobacco organs during wounding. Expression was induced more rapidly in upper, non-wounded leaves than in lower, wounded leaves. At 24 h after mechanical wounding, expression was tow in the roots, but increased in the stems and flowers, although to a lesser extent than in the leaves. At 3 or 10 d after insect-wounding, expression did not differ among organs, suggesting thatTobpreproHypSys- A could be induced globally and continuously throughout such stress. During that period, the activity of two defense proteins — PPO and PI — was consistent with the expression ofTobpreproHyp- Sys- A in various organs. This indicates that those proteins also could be regulated by TobHypSys, both globally and continuously.


Nicotiana tabacumpolyphenol oxidaseproteinase inhibitorsysteminwounding response

Copyright information

© The Botanical Society of Korea 2008

Authors and Affiliations

  1. 1.College of Life SciencesHuaZhong Normal UniversityWuhanChina