Journal of Plant Biology

, Volume 51, Issue 1, pp 35–41

Expressional characterization of dehydroascorbate reductase cDNA in transgenic potato plants

  • Young-Min Goo
  • Hyun Jin Chun
  • Tae-Won Kim
  • Cheol-Ho Lee
  • Mi-Jeong Ahn
  • Shin-Chul Bae
  • Kang-Jin Cho
  • Jae-An Chun
  • Chung-Han Chung
  • Shin-Woo Lee
Article

DOI: 10.1007/BF03030738

Cite this article as:
Goo, Y., Chun, H.J., Kim, T. et al. J. Plant Biol. (2008) 51: 35. doi:10.1007/BF03030738
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Abstract

In plants ascorbic acid (AsA) is a strong antioxidant or reductant that can be converted to dehydroascorbate (DHA) by oxidation. DHA, a very short-lived chemical, can either be hydrolyzed irreversibly to 2,3-diketogulonic acid or recycled to AsA by dehydroascorbate reductase (DHAR).DHAR cDNA, isolated from sesame hairy roots, was inserted into two plant expression vector syrtems with theCaMV35S promoter (CaMV35S : :DHAR) and a potato tuber-specific promoter,Patatin (Patatin : :DHAR). Southern and northern blot hybridization analyses indicated thatDHAR cDNA was successfully integrated into the potato genome and actively transcribed. High levels of sesameDHAR transcript and DHAR enzyme activity were determined, by thePatatin promoter, in regenerated potato tubers, but their levels in leaves were very low. In contrast, much higher amounts of transcript were accumulated in the leaves of CaMV35S : :DHAR regenerants than in the tubers while the activity of DHAR enzyme was higher in the latter. AsA content in the tubers of Patatin : :DHAR transgenic lines was also increased (1.1- to 1.3-fold) compared with that of non-transgenic plants. However, this was not true for the transgenic leaves. In contrast, theCaMV35S promoter was associated with AsA accumulations in both the tubers (up to 1.6-fold) and the leaves (up to 1.5-fold). However, more detailed analyses indicated that this increased enzyme activity was not always accompanied by an elevation in AsA content from transgenic plants. This suggests that other factors may limit the accumulation of vitamin C via ascorbate-recycling in transgenic potato plants.

Keywordsascorbic acidCaMV355 promoterdehydroascorbate (DHA)dehydroascorbate reductase (DHAR)Patatin promotersesame hairy roottransgenic potato

Copyright information

© The Botanical Society of Korea 2008

Authors and Affiliations

  • Young-Min Goo
    • 1
  • Hyun Jin Chun
    • 1
  • Tae-Won Kim
    • 1
  • Cheol-Ho Lee
    • 1
  • Mi-Jeong Ahn
    • 1
  • Shin-Chul Bae
    • 2
  • Kang-Jin Cho
    • 2
  • Jae-An Chun
    • 3
  • Chung-Han Chung
    • 3
  • Shin-Woo Lee
    • 1
  1. 1.Dept. of Crops BiotechnologyJinju National UniversityJinjuSouth Korea
  2. 2.National Institute of Agricultural BiotechnologySu-WonSouth Korea
  3. 3.Department of BiotechnologyDong-A UniversityBusanSouth Korea