Abstract
Since the late 1960s, researchers have observed that starch in the chloroplasts of the guard cells breaks down during the day and accumulates in the dark. Based on this, carbohydrates have historically been regarded as the primary osmotica modulating stomatal opening. However, the discovery of an important role for potassium uptake has led to the replacement of that starch-sugar hypothesis. Current research now focuses mainly on how K+ is transported in and out of cells when the stomata open or close. However, questions remain concerning photoreceptors, and the functioning of guard cell chloroplasts is still disputed. Coincidentally, some recent study results have again suggested that sucrose may play a major role in guard cell osmoregulation, thus supporting the original theory of starch-sugar involvement.
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Literature cited
Asai N, Nakazima N, Tamaoki M, Kamada H, Kondo N (2000) Role of malate synthesis mediated by phosphoenolpyruvate carboxylase in guard cells in the regulation of stomatal movements. Plant Cell Physiol: 10–15
Assmann SM, Zeiger E (1987) Guard cell bioenergetics,In E Zeiger, CD Farquhar, IR Cowan, eds, Stomatal Function. Stanford University Press, Stanford, CA, USA, 163–194
Doi M, Wada M, Shimazaki E (2006) The fernAdiantum capillusveneris lacks stomatal responses to blue light. Plant Cell Physiol:47: 748–755
Edwards MC, Smith GN, Bowling DJF (1988) Guard cells extrude protons prior to stomatal opening. J Exp Bot39: 1541–1547
Golow K, Taylor S, Zeiger E (1988) Photosynthetic carbon fixation in guard cell protoplasts fromVicia faba L: evidence from radiolabel experiments. Plant Physiol86: 700–705
Humble GD, Raschke K (1971) Stomatal opening quantitatively related to potassium transport. Plant Physiol48: 447–453
Hsiao TC (1976) Stomatal ion transport,In U Luttge, MG Pitman eds, Transport in Plants. Springer, Berlin, 195–221
Jamieson AP, Willmer CM (1984) Functional stomata in a variegated leaf chimera ofPelargonium zonale L. without guard cell chloroplasts. J Exp Bot35: 1053–1059
Kinoshila T, Doi M, Suelsugu N, Kagawa T, Wada M, Shimazaki K (2001) Phot 1 and Phot 2 mediate blue light regulation of stomatal opening. Nature414: 656–660
Lawson T, Oxborough K, James IL, Morison JIL, Baker NR (2002) Responses of photosynthetic electron transport in stomatal guard cells and mesophyll cells in intact leaves to light,CO 2 , and humidity. Plant Physiol128: 52–62
Lawson T, Oxborough K, Morison JIL, Baker NR (2003) The responses of guard and mesophyll cell photosynthesis toCO 2 , O2, light, and water stress in a range of species are similar. J Exp Bot54: 1 743–1752
Lee JS (2004) The effect of different light quality on the change membrane PD of the guard cell inTradescantia virginiana L. Kor J Environ Biol22: 89–92
Lee JS (2006) Response to red and blue lights by electrical currents on the surface of intact leaves. J Plant Biol49: 186–192
Lee JS, Bowling DJF (1992) Effect of the mesophyll on stomatal opening inCommelina communia. J Exp Bot43: 951–957
Lee JS, Bowling DJF (1993) Influence of the mesophyll on the change of electrical potential difference of guard cells induced by red light and CO2 inCommelina commuais L. and Trades-cantia virginiana L. Kor J Biol36: 383–389
Lee JS, Bowling DJF (1995) Influence of the mesophyll on stomatal opening. Aust J Plant Physiol22: 357–383
Lu P, Outlaw WH Jr, Smilh BG, Freed GA (1997) A new mechanism for the regulation of stomatal aperture size in intact leaves: Accumulation of mesophyll-derived sucrose in the guard cell wall ofVicia faba. Plant Physiol114: 109–118
MacRobbie EAC (1987) Ionic relation of guard cells,In E Zeiger, GD Farquhar, IR Cowan, eds, Stomatal Function. Stanford University Press, Stanford, CA, USA, 125–162
Mansfield TA (1986) The physiology of stomata: New insights into old problems,In JF Sutcliffe, FC Steward, JE Dale, eds, Plant Physiology. Academic Press, New York, 155–224
Nelson SD, Mayo JM (1975) The occurrence of functional nonchlorophyllous guard cells inPaphiopedilum spp. Can J Bot53: 1–7
Outlaw W (1989) Critical examination of the quantitative evidence for and againstCO 2 fixation by guard cells. Physiol Plant77: 275–281
Outlaw W (1996) Stomata: Biophysical and biochemical aspects,In NR Baker, ed, Photosynthesis and the Environment. Kluwer Academic Publishers, Dordrecht, The Netherlands, 241–259
Outlaw W, Manchester J (1979) Guard cell starch concentration quantitatively related to stomatal aperture. Plant Physiol64: 79–82
Poffenroth M, Green DB, Tallman G (1992) Sugar concentrations in guard cells ofVicia faba illuminated with red or blue light. Plant Physiol98: 1460–1471
Raschke K, Humble GD (1973) No uptake of ions required by opening stomata ofVicia faba. Planta115: 47–57
Reckmann U, Scheibe R, Raschke K (1990) Rubisco activity in guard cells compared with the solute requirement for stomatal opening. Plant Physiol92: 246–253
Ritte G, Sakr S, Rohrig R, Raschke K (1999) Rates of sugar uptake by guard cell protoplasts ofPisum sativum L related to the solute requirement for stomatal opening. Plant Physiol121: 647–655
Serrano E, Zeiger E, Hagiwan S (1988) Red light stimulates an electrogenic proton pump inVicia guard cell protoplasts. Proc Natl Acad Sci USA85: 436–440
Shabala SN (1998) Leaf bioelectric responses to rhythmical light: Identification of the contributions from stomatal and mesophyll cells. Aust J Plant Physiol24: 741–749
Taiz L, Zeiger E (2003) Plant Physiology, 3rd ed. Sinauer Associates, Sunderland, UK
Talboll LD, Zeiger E (1998) The role of sucrose in guard cell osmoregulation. J Exp Bot49: 329–337
Talboll LD, Shimayevich IJ, Chung Y, Hammad JW, Zeiger E (2003) Blue light and phytochrome-mediated stomatal opening in the npq1 and phot1 phot2 mutants ofArabidopsis. Plant Physiol133: 1522–1529
Tallman G, Zeiger E (1988) Light quality and osmoregulation inVicia guard cells: Evidence for involvement of three metabolic pathways. Plant Physiol88: 887–895
von Caemmerer S, Lawson T, Oxborough K, Baker NR, Andrews TJ, Raines CA (2004) Stomatal conductance does not correlate with photosynthetic capacity in transgenic tobacco with reduced amounts of Rubisco. J Exp Bot55: 1157–1166
Wong SC, Cowan IR, GD Farquhar (1978) Leaf conductance in relation to assimilation inEucalyptus pauciflora Sieb. ex Spreng. Plant Physiol62: 670–674
Zeiger E, Talbott LD, Frechilla S, Srivaslava A, Zhu J (2002) The guard cell chloroplast: A perspective for the twenty-first century. New Phytol153: 415–424
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Kim, D.J., Lee, J.S. Current theories for mechanism of stomatal opening: Influence of blue light; mesophyll cells, and sucrose. J. Plant Biol. 50, 523–526 (2007). https://doi.org/10.1007/BF03030704
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DOI: https://doi.org/10.1007/BF03030704