International Journal of Hematology

, 73:457

Hematopoietic Capability of CD34+ Cord Blood Cells: A Comparison With CD34+ Adult Bone Marrow Cells

Authors

  • Takahiro Ueda
    • Department of Pediatric Hematology/OncologyThe Institute of Medical Science, The University of Tokyo
    • Department of PediatricsNippon Medical School
  • Makoto Yoshida
    • Department of PediatricsHokkaido University School of Medicine
  • Hiroshi Yoshino
    • Department of Pediatric Hematology/OncologyThe Institute of Medical Science, The University of Tokyo
  • Kimio Kobayashi
    • Central Institute for Experimental Animals
  • Mariko Kawahata
    • Central Institute for Experimental Animals
  • Yasuhiro Ebihara
    • Department of Pediatric Hematology/OncologyThe Institute of Medical Science, The University of Tokyo
  • Mamoru Ito
    • Central Institute for Experimental Animals
  • Shigetaka Asano
    • Department of Pediatric Hematology/OncologyThe Institute of Medical Science, The University of Tokyo
    • Department of Hematology/OncologyThe Institute of Medical Science, The University of Tokyo
  • Tatsutoshi Nakahata
    • Department of PediatricsKyoto University
    • Department of Pediatric Hematology/OncologyThe Institute of Medical Science, The University of Tokyo
Rapid Communication

DOI: 10.1007/BF02994007

Cite this article as:
Ueda, T., Yoshida, M., Yoshino, H. et al. Int J Hematol (2001) 73: 457. doi:10.1007/BF02994007

Abstract

The characteristics of hematopoietic progenitor and stem cell (HPC/HSC) populations in mammals vary according to their ontogenic stage. In humans, HPC/HSCs from umbilical cord blood (CB) are increasingly used as an alternative to HPC/HSCs from adult bone marrow (BM) for the treatment of various hematologic disorders. How the hematopoietic activity of progenitor and stem cells in CB differs from that in adult BM remains unclear, however. We compared CD34+ cells, a hematopoietic cell population, in CB with those in adult BM using phenotypic subpopulations analyzed by flow cytometry, the colony-forming activity in methylcellulose clonal cultures, and the repopulating ability of these cells in NOD/Shi-scid (NOD/SCID) mice. Although the proportion of CD34+ cells was higher in adult BM than in CB mononuclear cells, the more immature subpopulations, CD34+CD33- and CD34+CD38- cells, were present in higher proportions in CD34+ CB cells. Clonal culture assay showed that more multipotential progenitors were present in CD34+ CB cells. When transplanted into NOD/SCID mice, CD34+ adult BM cells could not reconstitute human hematopoiesis in recipient BM, but CD34+ CB cells achieved a high level of engraftment, indicating that CD34+ CB cells possess a greater repopulating ability. These results demonstrated that human hematopoiesis changes with development from fetus to adult. Furthermore, CD34+ CB cells contained a greater number of primitive hematopoietic cells, including HSCs, than did adult BM, suggesting the usefulness of CD34+ CB cells not only as a graft for therapeutic HSC transplantation but also as a target cell population for ex vivo expansion of transplantable HSCs and for gene transfer in gene therapy.

Key words

Umbilical cord bloodCD34+ cellsColony-forming cellsNOD/SCID miceCord blood transplantation

Copyright information

© The Japanese Society of Hematology 2001