Journal of Biosciences

, Volume 25, Issue 1, pp 73–80

Avirulent mutants ofMacrophomina phaseolina andAspergillus fumigatus initiate infection inPhaseolus mungo in the presence of phaseolinone; levamisole gives protection

  • Suchandra Sett
  • Santosh K. Mishra
  • Kazia I. Siddiqui
Articles

DOI: 10.1007/BF02985184

Cite this article as:
Sett, S., Mishra, S.K. & Siddiqui, K.I. J. Biosci. (2000) 25: 73. doi:10.1007/BF02985184

Abstract

To evaluate the role of phaseolinone, a phytotoxin produced byMacrophomina phaseolina, in disease initiation, three nontoxigenic avirulent mutants of the fungus were generated by UV-mutagenesis. Two of them were able to initiate infection in germinatingPhaseolus mungo seeds only in the presence of phaseolinone. The minimum dose of phaseolinone required for infection in 30% seedlings was 2.5 (Μg/ml. A human pathogen,Aspergillus fumigatus was also able to infect germinating seeds of P.mungo in the presence of 5 (Μg/ml concentration of phaseolinone. Phaseolinone seemed to facilitate infection byA. fumigatus, which is not normally phytopathogenic, by reducing the immunity of germinating seedlings in a nonspecific way. Levamisole, a non-specific immunopotentiator gave protection against infection induced byA. fumigatus at an optimum dose of 50 (gmg/ml. Sodium malonate prevented the effects of levamisole.

Keywords

Aspergillusimmunopotentiatorinfectionlevamisolemacrophominaphaseolinoneprotection

Copyright information

© Indian Academy of Sciences 2000

Authors and Affiliations

  • Suchandra Sett
    • 1
  • Santosh K. Mishra
    • 1
  • Kazia I. Siddiqui
    • 1
  1. 1.Indian Institute of Chemical BiologyCalcuttaIndia