Avirulent mutants of Macrophomina phaseolina and Aspergillus fumigatus initiate infection in Phaseolus mungo in the presence of phaseolinone; levamisole gives protection
Received: 06 April 1999 Accepted: 07 September 1999 DOI:
10.1007/BF02985184 Cite this article as: Sett, S., Mishra, S.K. & Siddiqui, K.I. J. Biosci. (2000) 25: 73. doi:10.1007/BF02985184 Abstract
To evaluate the role of phaseolinone, a phytotoxin produced by
Macrophomina phaseolina, in disease initiation, three nontoxigenic avirulent mutants of the fungus were generated by UV-mutagenesis. Two of them were able to initiate infection in germinating Phaseolus mungo seeds only in the presence of phaseolinone. The minimum dose of phaseolinone required for infection in 30% seedlings was 2.5 (Μg/ml. A human pathogen, Aspergillus fumigatus was also able to infect germinating seeds of P. mungo in the presence of 5 (Μg/ml concentration of phaseolinone. Phaseolinone seemed to facilitate infection by A. fumigatus, which is not normally phytopathogenic, by reducing the immunity of germinating seedlings in a nonspecific way. Levamisole, a non-specific immunopotentiator gave protection against infection induced by A. fumigatus at an optimum dose of 50 (gmg/ml. Sodium malonate prevented the effects of levamisole. Keywords Aspergillus immunopotentiator infection levamisole macrophomina phaseolinone protection References
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