Effect of Granulocyte Colony-Stimulating Factor on Bone Metabolism During Peripheral Blood Stem Cell Mobilization
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Granulocyte colony—stimulating factor (G-CSF) has been shown to affect the biochemical markers of bone metabolism, including serum bone alkaline phosphatase (BALP), serum osteocalcin, and urine deoxypyridinoline. To determine the association between bone resorption and formation and the G-CSF—induced mobilization of peripheral blood stem cells (PBSC), we examined these markers during mobilization in 19 healthy donors. The average (± SEM) serum BALP level before treatment was 81.6 ± 17.0 IU/dL, and the level increased significantly to 117.7 ± 15.8 IU/dL on day 5 of G-CSF administration (P < .0001). The urine deoxypyridinoline level before treatment was 12.3 ± 2.4 nmol/mmol creatinine, and this level also increased significantly to 19.4 ± 3.0 nmol/mmol creatinine on day 5 of G-CSF administration (P < .0001). In contrast, the average level of serum osteocalcin significantly decreased from 8.07 ± 2.88 ng/mL to 1.53 ± 0.18 ng/mL on day 5 (P = .0353). During G-CSF administration, we also studied the serum levels of various cytokines (IL-lβ, osteoclastogenesis inhibitory factor [OCIF], IL-6, tumor necrosis factor a, transforming growth factor β, interferon-γ, macrophage colony—stimulating factor) related to bone metabolism. Only the kinetics of OCIF were significantly affected. The serum level of OCIF increased immediately after the start of G-CSF administration and remained high during G-CSF administration. These results demonstrate that high-dose G-CSF affects bone metabolism and that OCIF may play a role in bone metabolism. Consistent with the notion that G-CSF affects bone metabolism, a significant correlation was observed between CD34+ cell yield and the increase in urine deoxypyridinoline but not for the changes in serum BALP and osteocalcin levels. This result suggests that bone resorption is either directly or indirectly related to the mobilization of PBSC by G-CSF.
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- Effect of Granulocyte Colony-Stimulating Factor on Bone Metabolism During Peripheral Blood Stem Cell Mobilization
International Journal of Hematology
Volume 77, Issue 1 , pp 75-81
- Cover Date
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- Peripheral blood stem cells
- Bone metabolism
- Industry Sectors
- Author Affiliations
- 1. Department of Pediatrics, University ofTokushima School of Medicine, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan
- 2. Department of Pediatrics, Kyushu Cancer Center, Fukuoka
- 3. Department of Internal Medicine, National Cancer Center, Tokyo, Japan
- 4. Department of Pathology/Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA