Article

Archives of Pharmacal Research

, Volume 30, Issue 4, pp 475-480

Antithrombotic effects by oral administration of novel proteinase fraction from earthworm eisenia andrei on venous thrombosis model in rats

  • Chul Kyu LeeAffiliated withCentral Research Institute, Shin Poong Pharm. Co. Ltd. Email author 
  • , Jang Sik ShinAffiliated withCentral Research Institute, Shin Poong Pharm. Co. Ltd.
  • , Byung Su KimAffiliated withCentral Research Institute, Shin Poong Pharm. Co. Ltd.
  • , II Hwan ChoAffiliated withCentral Research Institute, Shin Poong Pharm. Co. Ltd.
  • , Young Shik KimAffiliated withNatural Products Research Institute, Seoul National University
  • , Eun Bang LeeAffiliated withCentral Research Institute, Shin Poong Pharm. Co. Ltd.Natural Products Research Institute, Seoul National University

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Abstract

A novel proteinase fraction, SPP-501, was purified from the earthworm, Eisenia andrei, and its antithrombotic effects compared with those of urokinase and t-PA (tissue type-plasminogen activator) in a thrombosis model, induced by the insertion of a stainless wire coil into the inferior vena cava. SPP-501, urokinase and t-PA were administrated once a day for 14 days. On the oral administration of SPP-501, as well as urokinase and t-PA, the thrombus weight was dramatically decreased. The euglobulin lysis time (ELT) was also shortened by SPP-501, but urokinase and t-PA failed to dissolve the euglobulin clot. Conversely, urokinase and t-PA produced detectable fibrinogen/fibrin degradation products (FDP), but SPP-501 did not. Thrombin induced platelet aggregation was desensitized in the SPP-501 treatment groups. With a high dose of SPP-501 (45 mg/kg), the APTT (activated partial thromboplastin time) was prolonged. These results suggest that SPP-501 shows both antithrombotic and fibrinolytic activities when orally administered.

Key words

Antithrombosis Fibrinolysis Lumbrokinases Venous thrombosis model