Archives of Pharmacal Research

, 27:177

Antioxidants and inhibitor of matrix metalloproteinase-1 expression from leaves ofzostera marina L.

  • Jin Hui Kim
  • Young Ho Cho
  • Sung Min Park
  • Kyung Eun Lee
  • Jeong Jae Lee
  • Bum Chun Lee
  • Hyeong Bae Pyo
  • Kyung Sik Song
  • Hum Dai Park
  • Yeo Pyo Yun
Research Articles Articles

DOI: 10.1007/BF02980103

Cite this article as:
Kim, J.H., Cho, Y.H., Park, S.M. et al. Arch Pharm Res (2004) 27: 177. doi:10.1007/BF02980103

Abstract

In order to develop new anti-photoaging agents, we examined the antioxidative activity and the inhibition effect of matrix metalloproteinase-1 (MMP-1) on the extracts of a marine product,Zostera marina L., which is known for its potent activity. Three compounds (compounds1,2, and3) were isolated from an ethyl acetate (EtOAc) soluble fraction of the product; they were identified as apigenin-7-O-β-D-glucoside (1), chrysoeriol (2), and luteolin (3). These compounds were found to scavenge radicals and reactive oxygen species (ROS) and were measured to have SC50 values of 0.18 mM, 0.68 mM, and 0.01 mM against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 0.04 mM, 0.03 mM, and 0.01 mM against the superoxide radical in the xanthine/xanthine oxidase system, respectively. Compound 3 suppressed the expression of MMP-1 by up to 44% at 4.0 μM and inhibited the production of interleukin 6 (IL-6), which is known as a cytokine that induces MMP-1 expression. From these results, compound3 and the other compounds were determined to have antioxidative activity and to inhibit MMP-1 expression. Thus, the three compounds are expected to be useful for preventing the photoaging of skin.

Key words

Zostera marina LAntioxidantMMP-1Apigenin-7-O-β-D-glucosideChrysoeriolLuteolin

Copyright information

© The Pharmaceutical Society of Korea 2004

Authors and Affiliations

  • Jin Hui Kim
    • 1
  • Young Ho Cho
    • 1
  • Sung Min Park
    • 1
  • Kyung Eun Lee
    • 1
  • Jeong Jae Lee
    • 1
  • Bum Chun Lee
    • 1
  • Hyeong Bae Pyo
    • 1
  • Kyung Sik Song
    • 2
  • Hum Dai Park
    • 3
  • Yeo Pyo Yun
    • 4
  1. 1.R&D Center, Hanbul Cosmetic CorporationSamsung-Myun, Umsung-Kun, ChungbukKorea
  2. 2.Dept. of Agricultural Chemistry, College of AgricultureKyungpook National UniversityTaeguKorea
  3. 3.Dept. of Biotechnology, College of EngineeringDaegu UniversityGyeongsanKorea
  4. 4.College of PharmacyChungbuk National UniversityCheongjuKorea