Archives of Pharmacal Research

, Volume 28, Issue 11, pp 1293–1301

Antiherpetic activities of flavonoids against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2)in vitro


DOI: 10.1007/BF02978215

Cite this article as:
Lyu, S.Y., Rhim, J.Y. & Park, W.B. Arch Pharm Res (2005) 28: 1293. doi:10.1007/BF02978215


Flavonoids, a group of low molecular weight phenylbenzopyrones, have various pharmacological properties including antioxidant, anticancer, bactericidal, and anti-inflammatory. We carried out anti-herpetic assays on 18 flavonoids in five classes and a virus-induced cytopathic effect (CPE) inhibitory assay, plaque reduction assay, and yield reduction assay were performed. When flavonoids were applied at various concentrations to Vero cells infected by HSV-1 and 2, most of the flavonoids showed inhibitory effects on virus-induced CPE. Among the flavonoids, EC, ECG (flavanols), genistein (isoflavone), naringenin (flavanone), and quercetin (flavonol) showed a high level of CPE inhibitory activity. The antiviral activity of flavonoids were also examined by a plaque reduction assay. EC, ECG., galangin, and kaempferol showed a strong antiviral activity, and catechin, EGC, EGCG., naringenin, chrysin, baicalin, fisetin, myricetin, quercetin, and genistein showed moderate inhibitory effects against HSV-1. In these experiments, flavanols and flavonols appeared to be more active than flavones. Furthermore, treatment of Vero cells with ECG and galangin (which previously showed strong antiviral activities) before virus adsorption led to a slight enhancement of inhibition as determined by a yield reduction assay, indicating that an intracellular effect may also be involved.

Key words

FlavonoidsHerpes simplex virus (HSV)Cytopathic effect (CPE)-inhibitory assaySelectivity index (SI)Plaque reduction assayYield reduction assayVero cells

Copyright information

© The Pharmaceutical Society of Korea 2005

Authors and Affiliations

  • Su -Yun Lyu
    • 1
  • Jee -Young Rhim
    • 2
  • Won -Bong Park
    • 2
  1. 1.Immune Modulation Research Group, The School of PharmacyUniversity of NottinghamUK
  2. 2.College of Natural SciencesSeoul Women’s UniversitySeoulKorea