Archives of Pharmacal Research

, Volume 30, Issue 1, pp 58–63

Isolation of limonoids and alkaloids fromPhellodendron amurense and their multidrug resistance (MDR) reversal activity

  • Yong Deuk Min
  • Hak Cheol Kwon
  • Min Cheol Yang
  • Kyu Ha Lee
  • Sang Un Choi
  • Kang Ro Lee
Articles Drug Development

DOI: 10.1007/BF02977779

Cite this article as:
Min, Y.D., Kwon, H.C., Yang, M.C. et al. Arch Pharm Res (2007) 30: 58. doi:10.1007/BF02977779

Abstract

Three limonoids and five alkaloids were isolated from the chloroform layer of the MeOH extract of the bark ofPhellodendron amurense (Rutaceae). The structures of the compounds isolated were determined to be obacunone (1), limonin (2), 12α-hydroxylimonin (3), γ-fagarine (4), oxyberberine (5), canthin-6-one (6), 4-methoxy-N-methyl-2-quinolone (7) and oxypalmatine (8) based on the physicochemical and spectroscopic data. Compounds3,5,7, and8 were first isolated from thePhellodendron amurense. The isolated compounds were then tested for their cytotoxicity against five human tumor cell linesin vitro using the SRB method. Compound5 showed significant cytotoxicity against the five tumor cell lines with ED50 values ranging from 0.30 to 3.0 μg/mL. The marginal or noncytotoxic compounds (1,2,3,4, and7) were examined for their P-gp related MDR reversal activities. Compound1 showed significant P-gp MDR inhibition activity in MES-SA/DX5 and HCT15 cells with an ED50 value of 0.028 μg/mL and 0.0011 μ.g/mL, respectively.

Key words

Phellodendron amurense Rutaceae Limonoid Alkaloid Multidrug resistance 

Copyright information

© The Pharmaceutical Society of Korea 2007

Authors and Affiliations

  • Yong Deuk Min
    • 1
  • Hak Cheol Kwon
    • 1
  • Min Cheol Yang
    • 1
  • Kyu Ha Lee
    • 1
  • Sang Un Choi
    • 2
  • Kang Ro Lee
    • 1
  1. 1.Natural Products Laboratory, College of PharmacySungkyunkwan UniversitySuwonKorea
  2. 2.Korea Research Institute of Chemical TechnologyDaejeonKorea