Research Articles Articles

Archives of Pharmacal Research

, 26:367

First online:

Neuraminidase Inhibitors fromReynoutria elliptica

  • Chu-Hyun LeeAffiliated withDivision of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Kyungpook National University
  • , Sang-ln KimAffiliated withDivision of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Kyungpook National University
  • , Kyung-Bok LeeAffiliated withCollege of Medicine, Konyang University
  • , Yung-Choon YooAffiliated withCollege of Medicine, Konyang University
  • , Si-Young RyuAffiliated withKorea Research Institute of Chemical Technology
  • , Kyung-Sik SongAffiliated withDivision of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Kyungpook National University Email author 

Abstract

In the course of screening neuraminidase inhibitors from herbal medicines, Reynoutria elliptica exhibited high inhibitory activity. Four active compounds were isolated from the ethyl acetate soluble fraction by consecutive purification using sillica gel, Sephadex LH-20 chromatography, and recrystallization. The chemical structures of these compounds were identified as 1,3,8-trihy-droxy-6-methylanthraquinone (emodin) 1,8-dihydroxy-3-methoxy-6-methylanthraquinone (emodin 3-methyl ether; physcion), 1,3,8-trihydroxy-6-hydoxymethylanthraquinone (ω-hydroxyemodin), and 3,5,4′-trihydroxystilbene (trans-resvertrol) by spectral data including MS,1 H-, and13 C-NMR. The IC50 values of emodin, emodin 3-methyl ether, ω-hydroxyemodin, andtrans-resvertrol were 2.81, 74.07, 10.49, and 8.77 μM, respectively. They did not inhibit other glycosidase such as glucosidase, mannosidase, and galactosidase, indicating that they were relatively specific inhibitors of neuraminidase.

Key words

Neuraminidase inhibitor Influenza Emodin Emodin 3-methyl ether Physcion ω-Hydroxy emodin trans-Resveratrol Reynoutria elliptica