Archives of Pharmacal Research

, 22:232

Aloesin and arbutin inhibit tyrosinase activity in a synergistic manner via a different action mechanism

  • Ying Hua Jin
  • Suk Jin Lee
  • Myung Hee Chung
  • Jeong Hill Park
  • Young In Park
  • Tae Hyeong Cho
  • Seung Ki Lee
Research Articles Biochemistry & Cell Biology

DOI: 10.1007/BF02976355

Cite this article as:
Jin, Y.H., Lee, S.J., Chung, M.H. et al. Arch Pharm Res (1999) 22: 232. doi:10.1007/BF02976355

Abstract

In this study, we present evidence that cotreatment of aloesin and arbutin inhibits tyrosinase activity in a synergistic manner by acting through a different action mechanism. Aloesin or arbutin similarly inhibited enzyme activity of human- and mushroom-tyrosinases with an IC50 value of 0.1 or 0.04 mM, respectively. Lineweaver-Burk plots of the enzyme kinetics data showed that aloesin inhibited tyrosinase activity noncompetitively with a Ki value of 5.3 mM, whereas arbutin did it competitively (Maeda, 1996). We then examined whether cotreatment of these agents inhibits the tyrosinase activity in a synergistic manner. The results showed that 0.01 mM aloesin in the presence of 0.03 mM arbutin inhibited activity of mushroom by 80% of the control value and the reverse was also true. The inhibitory effects were calculated to be synergistic according to the Bürgi method. Taken together, we suggest that aloesin along with arbutin inhibits in synergy melanin production by combined mechanisms of noncompetitive and competitive inhibitions of tyrosinase activity.

Key words

Synergistic tyrosinase inhibitorsAloesinArbutin

Copyright information

© The Pharmaceutical Society of Korea 1999

Authors and Affiliations

  • Ying Hua Jin
    • 1
  • Suk Jin Lee
    • 1
  • Myung Hee Chung
    • 2
  • Jeong Hill Park
    • 1
  • Young In Park
    • 3
  • Tae Hyeong Cho
    • 4
  • Seung Ki Lee
    • 1
  1. 1.College of PharmacySeoul National UniversitySeoulKorea
  2. 2.College of MedicineSeoul National UniversitySeoulKorea
  3. 3.College of Natural ResourcesKorea UniversitySeoulKorea
  4. 4.Namyang Aloe Bio-science Research InstituteYongjeong-RiKorea