Archives of Pharmacal Research

, Volume 23, Issue 6, pp 633–636

Identification of the calcium binding sites in translationally controlled tumor protein

  • Moonhee Kim
  • Yoonwha Jung
  • Kyunglim Lee
  • Choonmi Kim
Research Articles Biomedical Sciences

DOI: 10.1007/BF02975253

Cite this article as:
Kim, M., Jung, Y., Lee, K. et al. Arch Pharm Res (2000) 23: 633. doi:10.1007/BF02975253

Abstract

Translationally controlled tumor protein (TCTP), also known as lgE-dependent histamine-releasing factor, is a growth-related tumor protein. Although the primary sequence of rat TCTP does not reveal any recognizable Ca2+-binding motif, previous studies have demonstrated that rat TCTP consisting of 172 amino acids is a Ca2+-binding protein. However, the region of TCTP required for Ca2+ interaction has not been mapped to the molecule. Here, we reported that the Ca2+ binding region of TCTP, which was mapped by using a combination of deletion constructs of rat TCTP and45Ca2+-overlay assay, was confined to amino acid residues 81–112. This binding domain did not show any peculiar loop of calcium-binding motif such as CaLB domain and EF hand motif and it seems to be constituted of random coil regions neighboring the a helix. Thus, our data confirm that TCTP is a novel family of Ca2+-binding protein.

Key words

TCTP Calcium-binding sites 45Calcium-overlay assay 

Copyright information

© The Pharmaceutical Society of Korea 2000

Authors and Affiliations

  • Moonhee Kim
    • 1
    • 2
  • Yoonwha Jung
    • 1
    • 2
  • Kyunglim Lee
    • 1
    • 2
  • Choonmi Kim
    • 1
    • 2
  1. 1.College of Pharmacy, Centre for Cell Signaling ResearchEwha Womans UniversitySeoulKorea
  2. 2.Division of Molecular Life SciencesEwha Womans UniversitySeoulKorea

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