Articles Drug Development

Archives of Pharmacal Research

, 29:213

First online:

Protection of peroxynitrite-induced DNA damage by dietary antioxidants

  • Hye Kyung MoonAffiliated withSchool of Life Sciences and Biotechnology and Agro-Biotechnology Education Center, Kyungpook National University
  • , Eun Sun YangAffiliated withSchool of Life Sciences and Biotechnology and Agro-Biotechnology Education Center, Kyungpook National University
  • , Jeen Woo ParkAffiliated withSchool of Life Sciences and Biotechnology and Agro-Biotechnology Education Center, Kyungpook National University Email author 

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Abstract

The present study was undertaken to test the hypothesis that dietary antioxidants protect DNA damage induced by peroxynitrite, a potent physiological inorganic toxin. The present study showed that dietary antioxidants such as (−)-epigallocatechin, gallate, quercerin, rutin, resveratrol, and ursolic acid inhibit single strand breaks in supercoiled plasmid DNA induced by 3-morpholinosyndromine N-ethylcarbamide (SIN-1), a generator of peroxynitrite, through the reaction between nitric oxide and superoxide anion. The formation of 8-hydroxy-2′-deoxyguanosine (8-OH-dG) in calf thymus DNA by SIN-1 was also inhibited by dietary antioxidants. When U937 cells were incubated with 1 mM SIN-1 bolus, a significant increase of 8-OH-dG level was observed. However, oxidative DNA damage was significantly lower in the cells pre-treated with dietary antioxidants when cells were exposed to SIN-1.

Key words

Dietary antioxidants Peroxynitrite DNA damage