Serum neuron-specific enolase and immunohistochemical markers of neuroendocrine differentiation in lung cancer
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An enzyme immunoassay for serum neuron-specific enolase (NSE) was evaluated with respect to analytical performance and clinical utility and compared with immunohistochemical evaluation of neuroendocrine differentiation. Values obtained agreed well with values obtained using a radioimmunoassay method giving a correlation coefficient of 0.934. Analytical performance of the enzyme immunoassay was good but the diagnostic sensitivity of 82 % in extensive and 67% in limited disease was insufficient for serum NSE to be of value in the diagnosis of small cell lung cancer (SCLC). Serum NSE decreased significantly in 11 of 15 patients with SCLC following institution of chemotherapy. Classification of lung cancers into SCLC and non small cell lung cancer (NSCLC) types is largely based on tumour morphology. Neuroendocrine differentiation may not be morphologically evident. Immunohistochemical staining of tumour tissue with markers of neuroendocrine differentiation, i.e. NSE (both monoclonal and polyclonal antibodies) Leu 7, Chromogranin A and P G P 9.5 was performed in both patients with SCLC and NSCLC. 38 per cent of patients with NSCLC had both raised serum NSE and positive NSE (polyclonal) immunoperoxidase staining of lung tissue. A further 35 per cent of patients showed a raised serum NSE or positive immunohistochemistry but not both. The presence of two positive immunoperoxidase markers in lung tissue has been suggested as an indicator of responsiveness to chemotherapy in NSCLC patients. A number of factors may affect immunohistochemical positivity in tissue sections and the additional use of a serum marker may better define chemotherapy responsive groups.
Carney, D. N., Marangos, P. J. Ihdedc, Bunn, P. A. et al. Serum Neuron-Specific Enolase; a marker for disease extent and response to therapy of small cell lung cancer. The Lancet 1982; 13: 583–586.CrossRef
Lamb, D. Lung cancer and its classification. Anthony, P. P. and McSweeney, W. W. (Eds). Recent Advances in Histopathology. No. 13 Churchill Livingstone/RNM Edinburgh 1987; 13: 45–59.
Baylin, S. B. Neuroendocrine differentiation: A prognostic feature of non-small cell cancer. J. Clin. Oncol. 1989; 7: 1375–1376.PubMed
Visscher, D. W., Zarbo, R. J., Trojonawski, J. Q., Sakr, W., Crissman, J. D. Neuroendocrine differentiation on poorly differentiated lung carcinoma; a light microscopic and immunohistologic study. Mod. Pathol. 1990; 153: 508–512.
Bergh, J., Esscher, T., Steinholtz, L. et al. Immunocytochemical demonstration of neuron-specific enolase (NSE) in human lung cancers. Am. J. Clin. Pathol. 1985; 84: 1–7.PubMed
Linnoila, R. I., Mulshine, J. L., Steinberg, S. M. et al. Neuroendrine differentiation in endocrine and nonendocrine lung carcinomas. Am. J. Clin. Pathol. 1988; 90: 641–652.PubMed
Crompton, G. Small Cell Lung Cancer. BMJ 1990; 3000: 201–210.
Souhami, R.L. Neuroendocrine phenotype,chemosensitivity and prognosis in adenocarcinoma of the lung. Ann. of Oncol, 1991; 2: 323–324.
Graziano, S. L., Mazid, R., Newman, N. et al. The use of neuroendocrine immunoperoxidase markers to predict chemotherapy response in patients with non small cell lung cancer. J. Clin. Oncol. 1989; 7: 1398–1406.PubMed
Jorgensen, L. G. M., Hirsch, F. R., Skov, B. G. Osterlind, K., Cooper, E. H., Larsson, L. I. Occurence of neuronspecific enolase in tumour tissue and serum in small cell lung cancer. Br. J. Cancer. 1991; 63: 151.PubMed
Reidy, J. A., Skoczenski, B. A., Bleile, D. M., Usategui, M. An enzyme immunoassay for the detection of gamma-enolase in serum. Clin. Chem. 1987; 33: 928–929.
Cooper, E. H., Splinter, T. W. A., Brown, D. A., Muers, M. F., Peake, M. D., Pearson, S. L. Evaluation of a radioimmunoassay for Neuron Specific Enolase in Small Cell Lung Cancer. Br. J. Cancer 1985; 52: 333–338.PubMed
Hsu, S. M., Raine, L., Fanger, H. Use of Avidin-Biotin Peroxidase Complex (ABC) in immunohistochemical techniques. J. Histochem. Cytochem. 1981; 29: 577–580.PubMed
Johnson, D. H., Marangos, P. J., Forbes, J. et al. Potential utility of Neuron Specific Enolase levels in small cell carcinomas of the lung. Cancer Research 1984; 44: 5409–5414.PubMed
Teeling, M., McGing, P., Johnston, P., Carney, D. N. Clinical correlation of tumour endocrine markers in lung cancer. In: Hansen, H. H. (Eds) Basic and clinical concepts of lung cancer. Kluwer Academic Publishers 1989, chapter 9.
Carney, D. N. Biology of small cell lung cancer. The Lancet 1992; 339: 843–845.CrossRef
Esscher, T., Steinholtz, L., Bergh, T., Nou, E., Nilsson, K., Pahlman, S. Neuron Specific Enolase: A useful diagnostic serum marker for small cell carcinoma of the lung. Thorax. 1985; 40: 85–90.PubMed
Beasteall, G. H., Cooke, B., Rustin, G. J. S. et al. A review of the role of established tumour markers. Ann. Clin. Chem. 1991; 28: 5–18.
Jorgensen, L. G. M., Osterlind, K., Hansen, H. H. et al. The prognostic influence of serum neuron-specific enolase in small cell lung cancer. Br. J. Cancer 1988; 58: 805–807.PubMed
Watts, A. E., Said, J. W., Shintaku, I. P. et al. Chromogranin as a marker of neuroendocrine cells in cytologie material: An immunochemical study. Am. J. Clin. Pathol. 1985; 84: 273–277.
Bordi, C., Pilatio, F. P., D’Adda, T. Comparative study of seven neuroendocrine markers in pancreatic endocrine tumours. Virchows Archiv-A-Pathol. Anat. 1988; 413: 387–398.CrossRef
Thomas, P., Battifora, H., Mandenno, G.L.etal. Amonoclonal antibody against neuron-specific enolase. Am. J. Clin. Pathol. 1987; 88: 146–152.PubMed
Dhillon, A. P., Rode, J., Dhillon, D. P. et al. Neural markers in carcinoma of the lung. Br. J. Cancer 1985; 51: 645–652.PubMed
Harding, M., McAllister, J., Mulkis, G. et al. Neuronspecific enolase (NSE) in small cell lung cancer: A tumour marker of prognostic significance. Br. J. Cancer 1990; 61: 605–6PubMed
Skov, B. G., Sorensen, J. B., Hirsch, F. R. et al. Prognostic impact of histological demonstration of chromogranin A and neuron-specific enolase in pulmonary adenocarcinoma. Ann. Oncol. 1991; 2: 355–360.PubMed
- Serum neuron-specific enolase and immunohistochemical markers of neuroendocrine differentiation in lung cancer
Irish Journal of Medical Science
Volume 164, Issue 1 , pp 31-36
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- 1. Department of Biochemistry, St. Vincent’s Hospital, Dublin
- 2. Department of Histology, St. Vincent’s Hospital, Dublin
- 3. Department of Metabolism, St. Vincent’s Hospital, Dublin
- 4. Department of Oncology, St. Vincent’s Hospital, Dublin