, Volume 142, Issue 1, pp 230-243

Seasonal changes in the sensitivity of frog skin to prostaglandin and the effect of external sodium and chloride on the response

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Summary

It has been reported that prostaglandin E1 stimulates sodium transport across isolated frog skin. This paper is primarily concerned with a seasonal change in the sensitivity of frog skin to prostaglandin E1 and F1∝. To determine prostaglandin action on sodium transport, the abdominal skin ofRana temporaria was mounted in an Ussing-type chamber and the shortcircuit current was recorded. In some experiments the skin potential and conductance were also measured.

Arachidonic acid, a possible tissue precursor of prostaglandin also increases the short-circuit current. The prostaglandin action is independent of outside chloride and unchanged with 1/10 Ringer outside.

The frog skin shows the same seasonal variation in sensitivity to prostaglandins as reported by others for anti-diuretic hormone. Cold season frogs gave large responses whereas summer frogs at ambient temperatures were quite refractory. Pyruvate can restore, in part, the ability of summer skins to respond to prostaglandin as for anti-diuretic hormone.

It is concluded that the refractory nature of summer skins is due, in part, to a lack of a suitable energy source.