Biotechnology and Bioprocess Engineering

, 9:252

Enhanced production of succinic acid by metabolically engineeredEscherichia coli with amplified activities of malic enzyme and fumarase

Authors

  • Soon Ho Hong
    • Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering and Bioprocess Engineering Research CenterKorea Advanced Institute of Science and Technology
    • Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering and Bioprocess Engineering Research CenterKorea Advanced Institute of Science and Technology
    • Department of Biosystems and Bioinformatics Research CenterKorea Advanced Institute of Science and Technology
Article

DOI: 10.1007/BF02942339

Cite this article as:
Hong, S.H. & Lee, S.Y. Biotechnol. Bioprocess Eng. (2004) 9: 252. doi:10.1007/BF02942339

Abstract

Apfl ldhA double mutantEscherichia coli strain NZN111 was used to produce succinic acid by overexpressing theE. coli malic enzyme gene (sfcA). This strain, however, produced a large amount of malic acid as well as succinic acid. After the analyses of the metabolic pathways, thefumB gene encoding the anaerobic fumarase ofE. coli was co-amplified to solve the problem of malic acid accumulation. A plasmid, pTrcMLFu, was constructed, which contains an artificial operon (sfcA-fumB) under the control of the inducibletrc promoter. From the batch culture of recombinantE. coli NZN111 harboring pTrcMLFu, 7 g/L of succinic acid was produced from 20 g/L of glucose, with no accumulation of malic acid. From the metabolic flux analysis the strain was found under reducing power limiting conditions by severe reorientation of metabolic fluxes.

Keywords

succinic acidmalic enzymefumarasemetabolic engineering

Copyright information

© The Korean Society for Biotechnology and Bioengineering 2004