Applied Biochemistry and Biotechnology

, Volume 45, Issue 1, pp 407–415

Evaluation of the enzymatic susceptibility of cellulosic substrates using specific hydrolysis rates and enzyme adsorption

  • Dora Lee
  • Alex H. C. Yu
  • Ken K. Y. Wong
  • John N. Saddler
Session 2 Applied Biological Research

DOI: 10.1007/BF02941815

Cite this article as:
Lee, D., Yu, A.H.C., Wong, K.K.Y. et al. Appl Biochem Biotechnol (1994) 45: 407. doi:10.1007/BF02941815

Abstract

Adsorption of cellulases to cellulose is a critical step in the hydrolysis of cellulosic substrates. However, the importance of adsorption in determining the hydrolysis rate is unclear. The accessibility to cellulases and specific hydrolysis rates were measured for various substrates. No correlation was found between the amount of enzyme adsorbed and the initial hydrolysis rate for different substrates. Specific hydrolysis rates were found to differ among substrates. Furthermore, both accessibility to cellulases and the specific hydrolysis rate of substrates were found to be changed by chemical and physical pretreatment of the substrate.

Index Entries

Cellulasespecific hydrolysis rateadsorptioncellulosic substratescellulose hydrolysis

Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Dora Lee
    • 1
  • Alex H. C. Yu
    • 1
  • Ken K. Y. Wong
    • 1
  • John N. Saddler
    • 1
  1. 1.Forest Products Biotechnology, Faculty of ForestryUniversity of British ColumbiaCanada