Applied Biochemistry and Biotechnology

, Volume 57, Issue 1, pp 157–170

Conversion of lignocellulosics pretreated with liquid hot water to ethanol

Authors

  • G. Peter van Walsum
    • Thayer School of EngineeringDartmouth College
  • Stephen G. Allen
    • Hawaii Natural Energy InstituteUniversity of Hawaii at Manoa
  • Mark J. Spencer
    • Hawaii Natural Energy InstituteUniversity of Hawaii at Manoa
  • Mark S. Laser
    • Thayer School of EngineeringDartmouth College
  • Michael J. Antal
    • Hawaii Natural Energy InstituteUniversity of Hawaii at Manoa
  • Lee R. Lynd
    • Thayer School of EngineeringDartmouth College
Session 1 Thermal, Chemical, and Biological Processing

DOI: 10.1007/BF02941696

Cite this article as:
van Walsum, G.P., Allen, S.G., Spencer, M.J. et al. Appl Biochem Biotechnol (1996) 57: 157. doi:10.1007/BF02941696

Abstract

Lignocellulosic materials pretreated using liquid hot water (LHW) (220°C, 5 MPa, 120 s) were fermented to ethanol by batch simultaneous saccharification and fermentation (SSF) usingSaccharomyces cerevisiae in the presence ofTrichoderma reesei cellulase. SSF of sugarcane bagasse (as received), aspen chips (smallest dimension 3 mm), and mixed hardwood flour (−60 +70 mesh) resulted in 90% conversion to ethanol in 2–5 d at enzyme loadings of 15–30 FPU/g. In most cases, 90% of the final conversion was achieved within 75 h of inoculation. Comminution of the pretreated substrates did not affect the conversion to ethanol. The hydrolysate produced from the LHW pretreatment showed slight inhibition of batch growth ofS. cerevisiae. Solids pretreated at a concentration of 100 g/L were as reactive as those pretreated at a lower concentration, provided that the temperature was maintained at 220°C.

Index Entries

Liquid hot waterpretreatmentSSFinhibitionparticle size reduction

Copyright information

© Humana Press Inc. 1996