Mycotoxin Research

, 19:51

Analysis of deoxynivalenol and de-epoxy-deoxynivalenol in animal tissues by liquid chromatography after clean-up with an immunoaffinity column

  • H. Valenta
  • S. Dänicke
  • S. Döll
Article

DOI: 10.1007/BF02940093

Cite this article as:
Valenta, H., Dänicke, S. & Döll, S. Mycotox Res (2003) 19: 51. doi:10.1007/BF02940093

Abstract

A method for the determination of deoxynivalenol (DON) and its metabolite de-epoxy-deoxynivalenol (de-epoxy-DON) in blood serum, urine, faeces/digesta and bile fluid is described. Liquid samples (urine and bile after incubation with ß-glucuronidase) were extracted with ethyl acetate on a ChemElut column. Freeze dried faeces and digesta were extracted with a mixture of acetonitrile and water. For clean-up, serum and urine extracts could be directly applied to an immunoaffinity column (IAC). A pre-treatment prior to the IAC clean-up was necessary in the case of bile and faeces/digesta. DON and de-epoxy-DON were determined by high performance liquid chromatography (HPLC) with diode-array detection (DAD).

Because of the clean extracts, low detection limits in the range of 4 ng/ml (serum) to 20 ng/g (dried faeces/digesta) were achieved. The recovery of DON and de-epoxy-DON was in the range of 75–89% and 64–85%, respectively. DON was found in blood serum, urine, bile, digesta and faeces of pigs from several feeding studies with DON contaminated feed; de-epoxy-DON could be detected only in digesta, faeces and urine.

Keywords

mycotoxindeoxynivalenolde-epoxy-deoxynivalenolmethodanimal tissuesHPLC

Copyright information

© Society of Mycotoxin Research and Springer 2003

Authors and Affiliations

  • H. Valenta
    • 1
  • S. Dänicke
    • 1
  • S. Döll
    • 1
  1. 1.Institute of Animal NutritionFederal Agricultural Research Centre (FAL)BraunschweigGermany