Biotechnology and Bioprocess Engineering

, Volume 7, Issue 6, pp 371–374

Pilot scale production of poly(3-hydroxybutyrate-co-3-hydroxy-valerate) by fed-batch culture of recombinantEscherichia coli

  • Jong-il Choi
  • Sang Yup Lee
  • Kyungsup Shin
  • Woo Gi Lee
  • Si Jae Park
  • Ho Nam Chang
  • Yong Keun Chang
Article

DOI: 10.1007/BF02933524

Cite this article as:
Choi, J., Lee, S.Y., Shin, K. et al. Biotechnol. Bioprocess Eng. (2002) 7: 371. doi:10.1007/BF02933524

Abstract

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB/V)], by fed-batch culture of recombinantEscherichia coli harboring a plasmid containing theAlcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only. In a 30 L fermentor having aKLa value of 0.11 s−1, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively, giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having aKLa of 0.03 s−1, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively, giving a productivity of 1.06 g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinantE. coli in a large-scale fermentor having lowKLa value.

Keywords

P(3HB/V)recombinantE. colipilot-scale fermentoroxygen transfer rate

Copyright information

© The Korean Society for Biotechnology and Bioengineering 2002

Authors and Affiliations

  • Jong-il Choi
    • 1
    • 2
  • Sang Yup Lee
    • 1
    • 2
    • 3
  • Kyungsup Shin
    • 2
  • Woo Gi Lee
    • 2
  • Si Jae Park
    • 1
    • 2
  • Ho Nam Chang
    • 2
  • Yong Keun Chang
    • 2
  1. 1.Metabolic and Biomolecular Engineering National Research LaboratoryKorea Advanced Institute of Science and TechnologyDaejeonKorea
  2. 2.Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research CenterKorea Advanced Institute of Science and TechnologyDaejeonKorea
  3. 3.Center for Ultramicrochemical Process SystemsKorea Advanced Institute of Science and TechnologyDaejeonKorea