Article

Biotechnology and Bioprocess Engineering

, Volume 7, Issue 6, pp 371-374

First online:

Pilot scale production of poly(3-hydroxybutyrate-co-3-hydroxy-valerate) by fed-batch culture of recombinantEscherichia coli

  • Jong-il ChoiAffiliated withMetabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and TechnologyDepartment of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology
  • , Sang Yup LeeAffiliated withMetabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and TechnologyDepartment of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and TechnologyCenter for Ultramicrochemical Process Systems, Korea Advanced Institute of Science and Technology Email author 
  • , Kyungsup ShinAffiliated withDepartment of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology
  • , Woo Gi LeeAffiliated withDepartment of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology
  • , Si Jae ParkAffiliated withMetabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and TechnologyDepartment of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology
  • , Ho Nam ChangAffiliated withDepartment of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology
  • , Yong Keun ChangAffiliated withDepartment of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology

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Abstract

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB/V)], by fed-batch culture of recombinantEscherichia coli harboring a plasmid containing theAlcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only. In a 30 L fermentor having aK La value of 0.11 s−1, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively, giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having aK La of 0.03 s−1, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively, giving a productivity of 1.06 g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinantE. coli in a large-scale fermentor having lowK La value.

Keywords

P(3HB/V) recombinantE. coli pilot-scale fermentor oxygen transfer rate