Optimization of a chemically defined, minimal medium forClostridium thermosaccharolyticum
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
This article presents results from a systematic study aimed at for mulating a defined, minimal medium for the growth ofClostridium thermosaccharolyticum in batch and in continuous culture. At least one vitamin appears to be essential, and there is no demonstrable requirement for trace minerals. The defined medium is shown to support growth on high substrate concentrations with scaled nutrient levels and is expected to permit complete utilization when nutrient limitation(s) are overcome. The observed elemental requirements are compared with cell mass fraction measurements and with a typical cell composition. The maximum growth rate (μmax) for batch growth ofC thermosaccharolyticum on the minimal medium is 0.27h−1 as compared with values of ∼0.4 h−1 typically reported for growth on complex media. However, exponential growth terminates at an optical density of about 0.22 corresponding to about 40% of the final value attained. Greater understanding of nutrient requirements and interactions is needed to address this issue.
Wang, D. I. C., Avgerinos, G. C., Biocic, I., and Wang, S-D. (1983)Phil. Trans. Royal Soc. B300, 323–333.CrossRef
Slapack, G. E., Russell, I., and Stewart, G. G. (1987) inThermophilic Microbes in Ethanol Production, CRC, Boca Raton, FL, p. 74.
Carriera, L. H. and Ljundahl, L. G. (1984) inProduction of Ethanol from Biomass Using Anaerobic Thermophilic Bacteria in Liquid Fuel Developments, Wise, D. L., ed. CRC, Boca Raton, FL, pp. 1–30.
Lynd, L. R., Ahn, H.-J., Anderson, G., Hill, P., Kersey, D. S., and Klapatch, T. (1991)Appl. Biochem. Biotechnol. 28/29, 549–570.CrossRef
Sato, K., Tomita, M., Yonemura, S., Goto, S., Sekine, K., Okuma, E., Takagi, Y., Hon-nami, K., and Saiki, T. (1993),Biosci. Biotechnol. Biochem. 57(12), 2116–2121.
Venkateswaran, S. and Demain, A. L. (1986),Chem. Eng. Commun. 45, 53–60.CrossRef
BAskaran, S., Ahn, H.-J., and Lynd, L. R. (1994),Biotechnol. Prog. in press.
Hill, P. W., Klapatch, T. R., and Lynd, L. R. (1993)Biotechnol. Bioeng. 42, 873–883.CrossRef
Garcia-Martinez, D. V., Shinmyo, A., Madia, A., and Demain, A. L. (1980),Eur. J. Appl. Microbiol. Biotechnol. 9, 189–197.CrossRef
Johnson, E. A., Madia, A., and Demain, A. L. (1981)Appl. Environ. Microbiol. 41(4), 1060–2062.
Klapatch, T. R., Hogsett, D. A. L., Baskaran, S., and Pal, S. (1994),Appl. Biochem. Biotechnol. 45/46, 209–223.CrossRef
Bailey, J. E. and Ollis, D. F. (1986),Biochemical Engineering Fundamentals, 2nd ed. McGraw Hill, p. 28.
Pirt, J. S. (1975), inPrinciples of Microbe and Cell Cultivation. John Wiley, New York: p. 124.
- Optimization of a chemically defined, minimal medium forClostridium thermosaccharolyticum
Applied Biochemistry and Biotechnology
Volume 51-52, Issue 1 , pp 399-411
- Cover Date
- Print ISSN
- Online ISSN
- Humana Press
- Additional Links
- defined medium
- minimal medium
- Clostridium thermosaccharolyticum
- Industry Sectors