Biotechnology and Bioprocess Engineering

, Volume 10, Issue 6, pp 505–509

Electrical recognition of label-free oligonucleotides upon streptavidin-modified electrode surfaces

Authors

  • Jong Wan Park
    • The Institute of Scientific and Industrial ResearchOsaka University
  • Ho Sub Jung
    • The Institute of Scientific and Industrial ResearchOsaka University
    • Core Research for Evolutional Science and Technology (CREST)Japan Science and Technology Corporation (JST)
    • The Institute of Scientific and Industrial ResearchOsaka University
    • Core Research for Evolutional Science and Technology (CREST)Japan Science and Technology Corporation (JST)
  • Tomoji Kawai
    • The Institute of Scientific and Industrial ResearchOsaka University
    • Core Research for Evolutional Science and Technology (CREST)Japan Science and Technology Corporation (JST)
Article

DOI: 10.1007/BF02932285

Cite this article as:
Park, J.W., Jung, H.S., Lee, H.Y. et al. Biotechnol. Bioprocess Eng. (2005) 10: 505. doi:10.1007/BF02932285

Abstract

For the purpose of developing a direct label-free electrochemical detection system, we have systematically investigated the electrochemical signatures of each step in the preparation procedure, from a bare gold electrode to the hybridization of label-free complementary DNA, for the streptavidin-modified electrode. For the purpose of this investigation, we obtained the following pertinent data; cyclic voltammogram measurements, electrochemical impedance spectra and square wave voltammogram measurements, in Fe(CN)63−/Fe(CN)64− solution (which was utilized as the electron transfer redox mediator). The oligonucleotide molecules on the streptavidin-modified electrodes exhibited intrinsic redox activity in the ferrocyanide-mediated electrochemical measurements. Furthermore, the investigation of electrochemical electron transfer, according to the sequence of oligonucleotide molecules, was also undertaken. This work demonstrates that direct label-free oligonucleotide electrical recognition, based on biofunctional streptavidin-modified gold electrodes, could lead to the development of a new biosensor protocol for the expansion of rapid, cost-effective detection systems.

Keywords

direct electrochemical detectionlabel-free DNAstep-by-step procedurestreptavidinbiotin system

Copyright information

© The Korean Society for Biotechnology and Bioengineering 2005