Article

Biotechnology and Bioprocess Engineering

, Volume 10, Issue 6, pp 505-509

First online:

Electrical recognition of label-free oligonucleotides upon streptavidin-modified electrode surfaces

  • Jong Wan ParkAffiliated withThe Institute of Scientific and Industrial Research, Osaka University
  • , Ho Sub JungAffiliated withThe Institute of Scientific and Industrial Research, Osaka UniversityCore Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST)
  • , Hea Yeon LeeAffiliated withThe Institute of Scientific and Industrial Research, Osaka UniversityCore Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST) Email author 
  • , Tomoji KawaiAffiliated withThe Institute of Scientific and Industrial Research, Osaka UniversityCore Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST)

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Abstract

For the purpose of developing a direct label-free electrochemical detection system, we have systematically investigated the electrochemical signatures of each step in the preparation procedure, from a bare gold electrode to the hybridization of label-free complementary DNA, for the streptavidin-modified electrode. For the purpose of this investigation, we obtained the following pertinent data; cyclic voltammogram measurements, electrochemical impedance spectra and square wave voltammogram measurements, in Fe(CN)6 3−/Fe(CN)6 4− solution (which was utilized as the electron transfer redox mediator). The oligonucleotide molecules on the streptavidin-modified electrodes exhibited intrinsic redox activity in the ferrocyanide-mediated electrochemical measurements. Furthermore, the investigation of electrochemical electron transfer, according to the sequence of oligonucleotide molecules, was also undertaken. This work demonstrates that direct label-free oligonucleotide electrical recognition, based on biofunctional streptavidin-modified gold electrodes, could lead to the development of a new biosensor protocol for the expansion of rapid, cost-effective detection systems.

Keywords

direct electrochemical detection label-free DNA step-by-step procedure streptavidinbiotin system