Folia Microbiologica

, Volume 51, Issue 2, pp 141–145

Comparison of antagonistic ability against enteropathogens by G+ and G anaerobic dominant components of human fecal microbiota

  • D. A. Gomes
  • A. M. L. Souza
  • R. V. Lopes
  • A. C. Nunes
  • J. R. Nicoli
Article

DOI: 10.1007/BF02932170

Cite this article as:
Gomes, D.A., Souza, A.M.L., Lopes, R.V. et al. Folia Microbiol (2006) 51: 141. doi:10.1007/BF02932170

Abstract

To confirm if anaerobic G+-components are those responsible for the function of colonization resistance, obligate anaerobic G+- and G-bacteria from normal dominant microbiota of human feces were isolated from three successive collections and then used inin vitro assays for antagonism against two enteropathogenic bacteria. The production of inhibitory diffusible compounds was determined on supplemented BHI agar and MRS agar media for G- and G+-bacteria, respectively.Salmonella enterica subsp.enterica serovar Typhimurium andShigella sonnei were used as indicators. G+-bacteria presented a higher overall antagonistic frequency against both pathogenic bacteria (57 and 64 % forS. enterica serovar Typhimurium andS. sonnei, respectively) when compared to G+-microorganisms but with a quite elevated variation between volunteers (0–100 %) and collection samples (40–72 and 40–80 % forS. enterica sv. Typhimurium andS. sonnei, respectively). On the other hand, only three among 143 G-isolates tested showed antagonistic activity. The results showed that, at leastin vitro, obligate anaerobic G+-components of the dominant human fecal microbiota present a higher potential for antagonism against the enteropathogenic models tested than do G-bacteria.

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2006

Authors and Affiliations

  • D. A. Gomes
    • 1
  • A. M. L. Souza
    • 1
  • R. V. Lopes
    • 1
  • A. C. Nunes
    • 2
  • J. R. Nicoli
    • 1
  1. 1.Departmento de MicrobiologiaUniversidade Federal de Minas GeraisBelo Horizonte (MG)Brazil
  2. 2.Departamento de Biologia Geral, I.C.B.Universidade Federal de Minas GeraisBelo Horizonte (MG)Brazil