Folia Microbiologica

, Volume 52, Issue 1, pp 3–14

Response of the bioluminescent bioreporterPseudomonas fluorescens HK44 to analogs of naphthalene and salicylic acid

  • J. Trögl
  • G. Kuncová
  • L. Kubicová
  • P. Pařík
  • J. Hálová
  • K. Demnerová
  • S. Ripp
  • G. S. Sayler
Article

DOI: 10.1007/BF02932131

Cite this article as:
Trögl, J., Kuncová, G., Kubicová, L. et al. Folia Microbiol (2007) 52: 3. doi:10.1007/BF02932131

Abstract

Pseudomonas fluorescens HK44 is alux-based bioluminescent bioreporter capable of selective luminescence in the presence of naphthalene and/or salicylic acid intermediate of its metabolism. We attempted to induce bioluminescence (BL) in this strain with 72 compounds,viz. substituted naphthalenes, naphthalene-like compounds (e.g., quinoline), substituted salicylic acids, salicylic acid-like compounds (e.g., 2-anthranilic acid), oligocyclic aromates, and intermediates of naphthalene metabolism to better discriminate response specificity. From them, 42 induced BL significantly lower as compared to naphthalene, three (viz. isoquinoline,o-cresol, and salicylamide) induced BL significantly greater than naphthalene, and 27 yielded no bioluminescent response whatsoever. Strain HK44 is therefore not prone to extensive false-positive signaling and can serve as a fairly specific indicator organism for naphthalene bioavailability. At elevated concentrations, 41 compounds inhibited BL. Thus, the inclusion of constitutive bioreporter controls as indicators of sample toxicity is vital to successful biosensing application.

Abbreviations

AU

arbitrary unit(s)

PAH

oligocyclic aromates (‘polycyclic aromatic hydrocarbons’)

BL

bioluminescence

RBL

relative bioluminescence

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2007

Authors and Affiliations

  • J. Trögl
    • 1
  • G. Kuncová
    • 1
  • L. Kubicová
    • 2
  • P. Pařík
    • 3
  • J. Hálová
    • 4
  • K. Demnerová
    • 5
  • S. Ripp
    • 6
  • G. S. Sayler
    • 6
  1. 1.Institute of Chemical Process FundamentalsAcademy of Sciences of the Czech RepublicPragueCzechia
  2. 2.Department of Inorganic and Organic ChemistryFaculty of Pharmacy, Charles UniversityHradec KrálovéCzechia
  3. 3.Department of Organic Chemistry, Faculty of Chemical TechnologyUniversity of PardubicePardubiceCzechia
  4. 4.Institute of Inorganic ChemistryAcademy of Sciences of the Czech RepublicŘežCzechia
  5. 5.Department of Biochemistry and MicrobiologyInstitute of Chemical TechnologyPragueCzechia
  6. 6.Center for Environmental BiotechnologyUniversity of TennesseeKnoxvilleUSA
  7. 7.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPragueCzechia

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