, Volume 52, Issue 1, pp 3-14

Response of the bioluminescent bioreporterPseudomonas fluorescens HK44 to analogs of naphthalene and salicylic acid

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Pseudomonas fluorescens HK44 is alux-based bioluminescent bioreporter capable of selective luminescence in the presence of naphthalene and/or salicylic acid intermediate of its metabolism. We attempted to induce bioluminescence (BL) in this strain with 72 compounds,viz. substituted naphthalenes, naphthalene-like compounds (e.g., quinoline), substituted salicylic acids, salicylic acid-like compounds (e.g., 2-anthranilic acid), oligocyclic aromates, and intermediates of naphthalene metabolism to better discriminate response specificity. From them, 42 induced BL significantly lower as compared to naphthalene, three (viz. isoquinoline,o-cresol, and salicylamide) induced BL significantly greater than naphthalene, and 27 yielded no bioluminescent response whatsoever. Strain HK44 is therefore not prone to extensive false-positive signaling and can serve as a fairly specific indicator organism for naphthalene bioavailability. At elevated concentrations, 41 compounds inhibited BL. Thus, the inclusion of constitutive bioreporter controls as indicators of sample toxicity is vital to successful biosensing application.

This work was supported by theCzech Grant Foundation (grant no. 104/05/2637 and 203/06/1244), theMinistry of Education, Youth and Sports of the Czech Republic (projects MŠM 002 162 0822 and MŠM 002 162 7501) and theGrant Agency of the Academy of Sciences of the Czech Republic (research plan AV 0Z 4032 0502). The authors thank Prof. Milo< Tichý, DSc, for assistance.