Biotechnology and Bioprocess Engineering

, Volume 10, Issue 3, pp 262–269

A comparison of lyophilized amniotic membrane with cryopreserved amniotic membrane for the reconstruction of rabbit corneal epithelium

  • Jae-Il Ahn
  • In-Keun Jang
  • Doo-Hoon Lee
  • Young-Kwon Seo
  • Hee-Hoon Yoon
  • Youn-Ho Shin
  • Jae-Chan Kim
  • Kye-Yong Song
  • Hee-Gu Lee
  • Eun-Kyung Yang
  • Ki-Ho Kim
  • Jung-Keug Park
Article

DOI: 10.1007/BF02932023

Cite this article as:
Ahn, JI., Jang, IK., Lee, DH. et al. Biotechnol. Bioprocess Eng. (2005) 10: 262. doi:10.1007/BF02932023

Abstract

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn’s AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an explantation method. The corneal epithelium could be reconstructed by culturing the third-passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a CAM. The corneal epithelium reconstructed on the LAM and CAM, supported by the two-Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the CAM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a goodin vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

Keywords

corneaepitheliumamniotic membraneteflon ring

Copyright information

© The Korean Society for Biotechnology and Bioengineering 2005

Authors and Affiliations

  • Jae-Il Ahn
    • 1
  • In-Keun Jang
    • 1
    • 2
  • Doo-Hoon Lee
    • 1
  • Young-Kwon Seo
    • 1
  • Hee-Hoon Yoon
    • 1
  • Youn-Ho Shin
    • 1
  • Jae-Chan Kim
    • 2
  • Kye-Yong Song
    • 3
  • Hee-Gu Lee
    • 4
  • Eun-Kyung Yang
    • 5
  • Ki-Ho Kim
    • 5
  • Jung-Keug Park
    • 1
  1. 1.Department of Chemical and Biochemical EngineeringDongguk UniversitySeoulKorea
  2. 2.Department of OphthalmologyChung-Ang University HospitalSeoulKorea
  3. 3.Department of Pathology, College of MedicineChung-Ang UniversitySeoulKorea
  4. 4.Molecular and Cell Biology Research DivisionKorea Research Institute of Bioscience and BiotechnologyTae-JonKorea
  5. 5.R&D CenterBioland Ltd.Chun-AnKorea