Biotechnology and Bioprocess Engineering

, Volume 6, Issue 2, pp 75–88

Microbial production of riboflavin using riboflavin overproducers,Ashbya gossypii, Bacillus subtilis, andCandida famate: An overview


DOI: 10.1007/BF02931951

Cite this article as:
Lim, S.H., Choi, J.S. & Park, E.Y. Biotechnol. Bioprocess Eng. (2001) 6: 75. doi:10.1007/BF02931951


In this paper, the microbial production of riboflavin is reviewed and includes descriptions of riboflavin overproducers, and the biosynthesis and details of the key-enzyme genes related to riboflavin. Three kinds of riboflavin overproducers are known;Bacillus subtilis andCandida famate utilize glucose as a carbon source, but the fungusAshbya gossypii requires plant oil as its sole carbon source. The starting material in riboflavin biosynthesis is guanosine triphosphate (GTP), which is converted to riboflavin through six enzymatic reactions. ThoughBacillus subtilis, Candida famate, andAshbya gossypii operate via different pathways until GTP, they follow the same pathway from GTP to riboflavin. From the metabolic viewpoint, with respect to improved riboflavin production, the supplementation of GTP, a process-limiting precursor must be considered. The GTP fluxes originate from three sources, serine, threonine and glyoxylate cycles. The development of pathways to strengthen GTP supplementation using biotechnological techniques remains an issue for future research.


riboflavin Ashbya gossypii vitamin B2 metabolic engineering 

Copyright information

© The Korean Society for Biotechnology and Bioengineering 2001

Authors and Affiliations

  • Seong Han Lim
    • 1
  • Jong Soo Choi
    • 2
  • Enoch Y. Park
    • 1
  1. 1.Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of AgricultureShizuoka UniversityShizuokaJapan
  2. 2.Laboratory of Fermentation, R&D CenterBASF Co. Ltd.JeonBukKorea

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