Biotechnology and Bioprocess Engineering

, Volume 12, Issue 1, pp 9–14

Fabricating tissues: Analysis of farming versus engineering strategies

  • Umber Cheema
  • Showan N. Nazhat
  • Burçak Alp
  • Farhad Foroughi
  • Nelomi Anandagoda
  • Vivek Mudera
  • Robert A. Brown
Article

DOI: 10.1007/BF02931797

Cite this article as:
Cheema, U., Nazhat, S.N., Alp, B. et al. Biotechnol. Bioprocess Eng. (2007) 12: 9. doi:10.1007/BF02931797

Abstract

Tissue Engineering has expanded rapidly towards target applications of tissue repair and regeneration, whilst generating surprisingly novel models to study tissue modelling. However, clinical success in producing effective engineered tissues such as bone, skin, cartilage, and tendon, have been rare and limited. Problems tend to focus on how to stimulate the replacement of initial scaffold with mechanically functional, native extracellular matrix (principally collagen). Typical approaches have been to develop perfused and mechanically active bioreactors, with the use of native collagen itself as the initial scaffold, though the idea remains that cells do the fabrication (i.e. a cultivation process). We have developed a new, engineering approach, in which the final collagen template is fabricatedwithout cell involvement. The first part of this biomimetic engineering involves a plastic compression of cellular native collagen gels to form dense, strong, collagenous neotissues (in minutes). Further steps can be used to orientate and increase collagen fibril diameter, again by non-cell dependent engineering. This allows operator control of cell or matrix density and material properties (influencing biological half life and fate). In addition, this (non-cultivation) approach can incorporate techniques to generate localised 3D structures and zones at a meso-scale. In conclusion, the use of biomimetic engineering based on native collagen, rather than cell-cultivation approaches for bulk matrix fabrication, produces huge benefits. These include speed of fabrication (minutes instead of weeks and months), possibility of fine control of composition and 3D nano-micro scale structure and biomimetic complexity.

Keywords

collagen depositionplastic compression3D meso-structurecell densityfibril densitybiomimetic fabricationgel behaviour

Copyright information

© The Korean Society for Biotechnology and Bioengineering 2007

Authors and Affiliations

  • Umber Cheema
    • 1
  • Showan N. Nazhat
    • 2
  • Burçak Alp
    • 1
  • Farhad Foroughi
    • 1
  • Nelomi Anandagoda
    • 1
  • Vivek Mudera
    • 1
  • Robert A. Brown
    • 1
  1. 1.Tissue Repair and Engineering Centre, Institute of Orthopaedics, RNOHUniversity College London (UCL)LondonUK
  2. 2.Division of Biomaterials and Tissue EngineeringUCL Eastman Dental InstituteLondonUK
  3. 3.Department of Mining, Metals and Materials EngineeringMcGill UniversityMontrealCanada