Folia Microbiologica

, Volume 51, Issue 6, pp 547–554

Comparative studies of differential expression of chitinolytic enzymes encoded bychiA, chiB, chiC andnagA genes inAspergillus nidulans

  • T. Pusztahelyi
  • Z. Molnár
  • T. Emri
  • É. Klement
  • M. Miskei
  • J. Kerékgyártó
  • J. Balla
  • I. Pócsi
Article

DOI: 10.1007/BF02931619

Cite this article as:
Pusztahelyi, T., Molnár, Z., Emri, T. et al. Folia Microbiol (2006) 51: 547. doi:10.1007/BF02931619

Abstract

N-Acetyl-d-glucosamine, chito-oligomers and carbon starvation regulatedchiA, chiB, andnagA gene expressions inAspergillus nidulans cultures. The gene expression patterns of the main extracellular endochitinase ChiB and theN-acetyl-β-d-glucosaminidase NagA were similar, and the ChiB-NagA enzyme system may play a morphological and/or nutritional role during autolysis. Alterations in the levels of reactive oxygen species or in the glutathione-glutathione disulfide redox balance, characteristic physiological changes developing in ageing and autolyzing fungal cultures, did not affect the regulation of either the growth-relatedchiA or the autolysis-coupledchiB genes although both of them were down-regulated under diamide stress. The transcription of thechiC gene with unknown physiological function was repressed by increased intracellular superoxide concentration.

Abbreviations

CBD

chitin-binding domain

CV

coefficient of variance

DCM

dry cell mass

GlcNAc

N-acetyl-d-glucosamine

(GlcNAc)2

N,N′-diacetylchitobiose

(GlcNAc)3

N,N′,N″-triacetylchitotriose

GlcNAcase

β-N-acetylhexosaminidase (N-acetyl-β-d-glucosaminidase; EC 3.2.1.52)

GSH

glutathione

GSSG

glutathione disulfide

MSB

2-methyl-1,4-naphthoquinone sodium hydrogen sulfite (‘menadione sodium bisulfite’)

NCBI

National Center for Biotechnology Information

4NPGlcNAc

4-nitrophenylN-acetyl-β-d-glucosaminide

4NP(GlcNAc)2

4-nitrophenylN,N′-diacetyl-β-d-chitobioside

4NP(GlcNAc)3

4-nitrophenylN,N′,N″-triacetyl-β-d-chitotrioside

ORF

open reading frame

PMF

peptide mass fingerprint

PSD

post-source decay

ROS

reactive oxygen species

RT-PCR

real-time polymerase chain reaction

SD

standard deviation

SDS

sodium dodecyl sulfate

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2006

Authors and Affiliations

  • T. Pusztahelyi
    • 1
  • Z. Molnár
    • 1
  • T. Emri
    • 1
  • É. Klement
    • 3
  • M. Miskei
    • 1
  • J. Kerékgyártó
    • 2
  • J. Balla
    • 4
  • I. Pócsi
    • 1
  1. 1.Department of Microbial Biotechnology and Cell BiologyUniversity of DebrecenDebrecenHungary
  2. 2.Department of Biochemistry, Faculty of ScienceUniversity of DebrecenDebrecenHungary
  3. 3.Protecomics Research GroupBiological Research CenterSzegedHungary
  4. 4.Department of Nephrology, Faculty of MedicineUniversity of DebrecenDebrecenHungary