Effect of antioxidants onSaccharomyces cerevisiae mutants deficient in superoxide dismutases
- Cite this article as:
- Krasowska, A., Dziadkowiec, D., Łukaszewicz, M. et al. Folia Microbiol (2003) 48: 754. doi:10.1007/BF02931509
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S. cerevisiae strain Δsod1 lacking Cu,Zn-superoxide dismutase and Δsod1Δsod2 mutant lacking both Cu,Zn-SOD and Mn-superoxide dismutase displayed strongly reduced aerobic growth on glucose, glycerol and lactate; Δsod2 deletion had no effect on aerobic growth on glucose and largely precluded growth on glycerol and lactate. The oxygen-induced growth defects and their alleviation by antioxidants depended on growth conditions, in particular on oxygen supply to cells. Under strong aeration, vitamins A and E had a low effect, 100 µmol/L quercetin alleviated the growth defects of all three mutants while β-carotene had no growth-restoring effect. The superoxide producer paraquat inhibited the aerobic growth of all three mutants in a concentration-dependent manner. Low concentrations of antioxidants had no effect on paraquat toxicity while higher concentrations supported the toxic effect of the agent.