, Volume 48, Issue 6, pp 754-760

Effect of antioxidants onSaccharomyces cerevisiae mutants deficient in superoxide dismutases

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Abstract

S. cerevisiae strain Δsod1 lacking Cu,Zn-superoxide dismutase and Δsod1Δsod2 mutant lacking both Cu,Zn-SOD and Mn-superoxide dismutase displayed strongly reduced aerobic growth on glucose, glycerol and lactate; Δsod2 deletion had no effect on aerobic growth on glucose and largely precluded growth on glycerol and lactate. The oxygen-induced growth defects and their alleviation by antioxidants depended on growth conditions, in particular on oxygen supply to cells. Under strong aeration, vitamins A and E had a low effect, 100 µmol/L quercetin alleviated the growth defects of all three mutants while β-carotene had no growth-restoring effect. The superoxide producer paraquat inhibited the aerobic growth of all three mutants in a concentration-dependent manner. Low concentrations of antioxidants had no effect on paraquat toxicity while higher concentrations supported the toxic effect of the agent.

The work was supported by thePolish State Committee for Scientific Research (KBN) grant 5PO 6A 02319, theGrant Agency of the Academy of Sciences of the Czech Republic (grant S5020202),Ministry of Education, Youth and Sports of the Czech Republic (grants CZE 01-032 and ME577), theInstitutional Research Project AV 0Z 502 0903 and grant 23/40 of theCzech-Polish Treaty on Scientific and Scientific-Technical Cooperation.