Folia Microbiologica

, Volume 48, Issue 6, pp 754–760

Effect of antioxidants onSaccharomyces cerevisiae mutants deficient in superoxide dismutases

  • A. Krasowska
  • D. Dziadkowiec
  • M. Łukaszewicz
  • K. Wojtowicz
  • K. Sigler
Article

DOI: 10.1007/BF02931509

Cite this article as:
Krasowska, A., Dziadkowiec, D., Łukaszewicz, M. et al. Folia Microbiol (2003) 48: 754. doi:10.1007/BF02931509

Abstract

S. cerevisiae strain Δsod1 lacking Cu,Zn-superoxide dismutase and Δsod1Δsod2 mutant lacking both Cu,Zn-SOD and Mn-superoxide dismutase displayed strongly reduced aerobic growth on glucose, glycerol and lactate; Δsod2 deletion had no effect on aerobic growth on glucose and largely precluded growth on glycerol and lactate. The oxygen-induced growth defects and their alleviation by antioxidants depended on growth conditions, in particular on oxygen supply to cells. Under strong aeration, vitamins A and E had a low effect, 100 µmol/L quercetin alleviated the growth defects of all three mutants while β-carotene had no growth-restoring effect. The superoxide producer paraquat inhibited the aerobic growth of all three mutants in a concentration-dependent manner. Low concentrations of antioxidants had no effect on paraquat toxicity while higher concentrations supported the toxic effect of the agent.

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2003

Authors and Affiliations

  • A. Krasowska
    • 1
  • D. Dziadkowiec
    • 1
  • M. Łukaszewicz
    • 1
  • K. Wojtowicz
    • 1
  • K. Sigler
    • 2
  1. 1.Institute of Genetics and MicrobiologyWroclaw UniversityWroclawPoland
  2. 2.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPragueCzechia