Susceptibility ofEscherichia coli to C2-C18 fatty acids
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
The antimicrobial activity of C2–C18 fatty acids was determinedin vitro in cultures of two strains ofEscherichia coli grown on glucose. Antimicrobial activity was expressed as IC50 (a concentration at which only 50 % of the initial glucose in the cultures was utilized). Utilization of glucose was inhibited by caprylic acid (IC50 0.30–0.85 g/L) and capric acid (IC50 1.25–2.03 g/L). Neither short-chain fatty acids (C2–C6) nor fatty acids with longer chain (C12–C18) influenced substrate utilization. Caproic acid, however, decreased cell yield in cultures ofE. coli in a dose-dependent manner. No inhibition of glucose utilization was produced with unsaturated fatty acids, oleic and linoleic. Calcium ions added in excess reversed the antimicrobial effect of capric acid, but not that of caprylic acid. Antimicrobial activity of caprylic and capric acid decreased when the bacteria were grown in the presence of straw particles, or repeatedly subcultured in a medium containing these compounds at low concentrations. Counts of viable bacteria determined by plating decreased after incubation with caprylic and capric acid (30 min; 1 g/L) at pH 5.2 from >109 to ≈102/mL. A reduction of a mere 0.94–1.96 log10 CFU was observed at pH 6.5–6.6. It can be concluded that caprylic acid, and to a lesser extent also capric acid, has a significant antimicrobial activity towardE. coli. Effects of other fatty acids were not significant or absent.
- Bearson S., Bearson B., Foster J.W.: Acid stress responses in enterobacteria.FEMS Microbiol.Lett. 147, 173–180 (1997). CrossRef
- Cañas-Rodriguez A., Smith H.W.: The identification of the antimicrobial factors of the stomach contents of sucking rabbits.Biochem.J. 100, 79–82 (1966).
- Cherrington C.A., Hinton M., Chopra I.: Effect of short-chain organic acids on macromolecular synthesis inEscherichia coli.J.Appl.Bacteriol. 68, 69–74 (1990).
- Clark B., Holms W.H.: Control of the sequential utilization of glucose and fructose byEscherichia coli.J.Gen.Microbiol. 95, 191–201 (1976).
- Dibner J.J., Buttin P.: Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism.J.Appl. Poultry Res. 11, 453–463 (2002).
- Galbraith H., Miller T.B., Paton A.M., Thompson J.K.: Antibacterial activity of long chain fatty acids and the reversal with calcium. magnesium. ergocalciferol and cholesterol.J.Appl.Bacteriol. 34, 803–813 (1971).
- Harfoot C.G., Noble R.C., Moore J.H.: The role of plant particles, bacteria and cell-free supernatant fractions of rumen contents in the hydrolysis of trilinolein and the subsequent hydrogenation of linoleic acid.Antonie van Leeuwenhoek 41, 533–542 (1975). CrossRef
- Hassinen J.B., Durbin G.T., Bernhart F.W.: The bacteriostatic effect of saturated fatty acids.Arch.Biochem.Biophys. 31, 183–189 (1951). CrossRef
- Jalč D., Kišidayová S., Nerud F.: Effect of plant oils and organic acids on rumen fermentationin vitro.Folia Microbiol. 47, 171–178 (2002). CrossRef
- Jenkins T.C., Palmquist D.L.: Effect of added fat and calcium onin vitro formation of insoluble fatty acid soaps and cell wall digestibility.J.Anim.Sci. 55, 957–963 (1982).
- Lauková A., Mareková M.: Production of bacteriocins by different enterococcal isolates.Folia Microbiol. 46, 49–50 (2001). CrossRef
- Maczulak A.E., Dehority B.A., Palmquist D.L.: Effects of long-chain fatty acids on growth of rumen bacteria.Appl.Environ.Microbiol. 42, 856–862 (1981).
- Marounek M., Skřivanová V., Savka O.G.: Effect of caprylic, capric and oleic acid on growth of rumen and rabbit cecal bacteria.J.Anim.Feed Sci. 11, 507–516 (2002).
- Morovský M., Pristaš P., Javorský P.: Bacteriocins of ruminal bacteria.Folia Microbiol. 46, 61–62 (2001). CrossRef
- Mortensen F.V., Moller J.K., Hessov I.B.: Effects of short-chain fatty acids onin vitro bacterial growth ofBacteroides fragilis andEscherichia coli.Acta Pathol.Microbiol.Immunol.Scand. 107, 240–244 (1999).
- Nieman C.: Influence of trace amounts of fatty acids on the growth of microorganisms.Bacteriol.Rev. 18, 147–163 (1954).
- Pantev A., Kabadjova P., Dalgalarrondo M., Haertlé T., Ivanova I., Dousset X., Prévost H., Chobert J.-M.: Isolation and partial characterization of an antibacterial substance produced byEnterococcus faecium.Folia Microbiol. 47, 391–400 (2002). CrossRef
- Prohászka L.: Antibacterial effect of short chain fatty acids in entericE. coli-infections of rabbits.Zbl.Vet.Med. B27, 631–639 (1980).
- Ricke S.C.: Perspectives on the use of organic acids and short chain fatty acids as antimicrobials.Poultry Sci. 82, 632–639 (2003).
- Sprong R.C., Hulstein M.F.E., Van der Meer R.: Bactericidal activities of milk lipids.Antimicrob.Agents Chemother. 45, 1298–1301 (2001). CrossRef
- Wallace R.J., Falconer M.L., Bhargava P.K.: Toxicity of short chain fatty acids at rumen pH prevents enrichment ofEscherichia coli by sorbitol in rumen contents.Curr.Microbiol. 19, 277–281 (1989). CrossRef
- Susceptibility ofEscherichia coli to C2-C18 fatty acids
Volume 48, Issue 6 , pp 731-735
- Cover Date
- Print ISSN
- Online ISSN
- Springer Netherlands
- Additional Links
- Industry Sectors
- Author Affiliations
- 1. Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 104 00, Prague-Uhřiněves, Czechia
- 2. Research Institute of Animal Production, 104 01, Prague-Uhřiněves, Czechia
- 4. Department of Microbiology and Biotechnology, Czech University of Agriculture in Prague, 165 21, Prague, Czechia