Article

Folia Microbiologica

, Volume 50, Issue 6, pp 519-523

The virulence markers ofSalmonella enterica serovar Typhimurium. Different phage-type strains isolated in Slovakia

  • V. MajtánAffiliated withDepartment of Microbiology, Slovak Medical University
  • , L’. MajtánováAffiliated withDepartment of Microbiology, Slovak Medical University
  • , M. SzabóováAffiliated withDepartment of Microbiology, Slovak Medical University

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Abstract

Phage-typing determination of cell-surface hydrophobicity, motility, and serovar-specific virulence plasmid was performed in a collection of 154 clinical isolates ofS. enterica serovar Typhimurium (SeT) isolated in Slovakia. All isolates were also examined in PCR for the presence of bothstn (enterotoxin) andiroB (siderophore) genes. The DT104 was the definitive phage type most frequently identified (37.7 %), the second most frequently isolated phage type was DT41 (5.8 %); the occurrence of other phage types was not epidemiologically significant. On the basis of virulence-marker investigation, 46.1 % of isolates were hydrophobic in the assay of bacterial adherence to xylene, and 97.4 % were hydrophobic in salt-aggregation test. Motility of more than 50 mm was expressed by 20.8 % isolates. The serovar-specific 90-kb virulence plasmid was contained in 138 (89.6 %) of isolates. AllSeT isolates were found (according to PCR) to carry theSalmonella-enterotoxin (stn) gene and the siderophore (iroB) gene. The increasing incidence ofSeT DT104 human strains in Slovakia requires continuous attention; this can be markedly improved by surveillance efficiency and made possible by determining relationships between sporadic isolates.