Session 3-Original Papers

Applied Biochemistry and Biotechnology

, Volume 18, Issue 1, pp 159-174

First online:

Digestion of pretreated aspen substrates

Hydrolysis rates and adsorptive loss of cellulase enzymes
  • K. TatsumotoAffiliated withFermentation Section, Biotechnology Research Branch, Solar Fuels Research Division, Solar Energy Research Institute
  • , J. O. BakerAffiliated withFermentation Section, Biotechnology Research Branch, Solar Fuels Research Division, Solar Energy Research Institute
  • , M. P. TuckerAffiliated withFermentation Section, Biotechnology Research Branch, Solar Fuels Research Division, Solar Energy Research Institute
  • , K. K. OhAffiliated withFermentation Section, Biotechnology Research Branch, Solar Fuels Research Division, Solar Energy Research Institute
  • , A. MohagheghiAffiliated withFermentation Section, Biotechnology Research Branch, Solar Fuels Research Division, Solar Energy Research Institute
  • , K. GrohmannAffiliated withFermentation Section, Biotechnology Research Branch, Solar Fuels Research Division, Solar Energy Research Institute
  • , M. E. HlmmelAffiliated withFermentation Section, Biotechnology Research Branch, Solar Fuels Research Division, Solar Energy Research Institute

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Abstract

Considerable controversy exists concerning the role lignin plays in the adsorption of cellulase enzymes on biomass. Recent studies using extracted, purified hardwood lignin have shown these materials have a propensity for cellulase adsorption; however, native lignin is carbohydrate-linked and far less condensed. In this study, we report the results of adsorption-kinetics analyses of cellulase-complex activities using five pretreated aspen substrates, including an exhaustively enzyme-hydrolyzed one. These data indicate that the polymer-binding cellulase activities are removed from solution at higher rates and extents in the presence of low lignin-content versus high lignin-content substrates. This order of adsorption was found to be essentially the inverse for beta-glucosidase adsorption.

Index Entries

Cellulase adsorption aspen lignin beta-glucosidase