International Journal of Pancreatology

, Volume 11, Issue 2, pp 109–116

HPLC and FPLC

Recent progress in the use of automated chromatography systems for resolution of pancreatic secretory proteins

Authors

  • Burkhard Göke
    • Zentrum Innere MedizinAbt. Gastroenterologie der Philipps Universität Marburg
  • Volker Keim
    • Medizinische Klinik IV, GastroenterologieKlinikum Mannheim, Universität Heidelberg
Article

DOI: 10.1007/BF02925982

Cite this article as:
Göke, B. & Keim, V. Int. J. Pancreatol. (1992) 11: 109. doi:10.1007/BF02925982

Summary

Automated chromatography techniques such as high-performance (high-pressure) liquid chromatography (HPLC) or fast protein liquid chromatography (FPLC) represent a promising, new approach toward resolving the complex protein mixture of pancreatic juice. To achieve highly reproducible and rapid resolution of pancreatic protein profiles, reversed-phase, hydrophobic interaction, and ion-exchange techniques are employed. Until now, none of the respectively reported methods has provided a convenient, specific procedure for diagostic purposes. However, experimental data prove that these methods are very sensitive and furthermore, easy to handle. Therefore, it seems possible to utilize such methods for diagnostics in future.

Key Words

Pancreatic juiceenzymesHPLCFPLC

Copyright information

© Humana Press Inc. 1992