Article

International Journal of Pancreatology

, Volume 11, Issue 2, pp 109-116

HPLC and FPLC

Recent progress in the use of automated chromatography systems for resolution of pancreatic secretory proteins
  • Burkhard GökeAffiliated withZentrum Innere Medizin, Abt. Gastroenterologie der Philipps Universität Marburg
  • , Volker KeimAffiliated withMedizinische Klinik IV, Gastroenterologie, Klinikum Mannheim, Universität Heidelberg

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Summary

Automated chromatography techniques such as high-performance (high-pressure) liquid chromatography (HPLC) or fast protein liquid chromatography (FPLC) represent a promising, new approach toward resolving the complex protein mixture of pancreatic juice. To achieve highly reproducible and rapid resolution of pancreatic protein profiles, reversed-phase, hydrophobic interaction, and ion-exchange techniques are employed. Until now, none of the respectively reported methods has provided a convenient, specific procedure for diagostic purposes. However, experimental data prove that these methods are very sensitive and furthermore, easy to handle. Therefore, it seems possible to utilize such methods for diagnostics in future.

Key Words

Pancreatic juice enzymes HPLC FPLC