Biologia Plantarum

, Volume 35, Issue 2, pp 169–177

Interspecific hybridization inBrassica: Application of flow cytometry for analysis of ploidy and genome composition in hybrid plants


  • P. S. Sabharwal
    • Department of Genetics and Plant BreedingUniversity of Agriculture
  • J. Doležel
    • Department of Genetics and Plant BreedingUniversity of Agriculture

DOI: 10.1007/BF02925934

Cite this article as:
Sabharwal, P.S. & Doležel, J. Biol Plant (1993) 35: 169. doi:10.1007/BF02925934


Interspecific hybrids from the crosses betweenBrassica campestris, B. carinata, B. juncea andB. napus were obtained throughin vitro ovary and ovule culture. F1 hybrids were studied morphologically and flow cytometry was used to estimate 2C nuclear DNA content both in parentalBrassica species and their hybrids. It was found that in comparison with the A genome, the B and the C genomes ofBrassica contained 26.9 % and 43.9 % more DNA, respectively. This finding may be used to distinguish interspecific hybrids containing various genome combinations. It was concluded that flow cytometric analysis of nuclear DNA content might be useful tool inBrassica breeding.

Copyright information

© Institute of Experimental Botany 1993