, Volume 37, Issue 2, pp 201-207

Synergism betweenClostridiwn Thermocellum cellulases cloned inEscherichia coli

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Abstract

We have obtained a synergistic effect during degradation of Avicel and filter paper byClostridium thermocellum cellulases (two endoglu-canases and one cellobiohydrolase) cloned inEscherichia coli. The highest degree of synergism was found at early stages of reaction, during the first 20 h: 2.5 and 2.9 on Avicel and filter paper, respectively. During combined action of all three cellulases the main product is cellobiose.