Micropropagation ofAsparagus cooperi as affected by growth regulators
- Cite this article as:
- Ghosh, B. & Sen, S. Biol Plant (1994) 36: 527. doi:10.1007/BF02921173
For clonal propagation ofAsparagus cooperi, shoot tips and node explants of 7, 20 and 35 d old spear from the region within 10 cm and below 25 cm from the apex were cultured in modified Murashige and Skoog's (1962) medium containing 6-benzylaminopurine (BAP). The required concentration of BAP varied in explants of different ages and types. In shoot tip culture, the rate of shoot multiplication was higher after 40 d than 60 d of culture. The maximum mumber (62–65) of shoots were obtained from shoot tip explants of 20 d old spear in the medium containing 2.0 mg dm−3 of BAP, 80 mg dm−3 of adenine and 0.02 mg dm−3 of α-naphthalene acetic acid after 60 d of culture. From node cultures, high number of shoots were obtained after 30 d. Pretreatment with BAP in liquid medium for 48 h was effective for semirejuvenescence. The individual shoots produced roots in presence of indole-3-butyric acid and also in potassium salt of indole-3-butyric acid, the later being more effective. All regeneratns were cytologically stable.
potassium salt of indole-3-butyric acid
α-naphthalene acetic acid
- MS medium
medium of Murashige and Skoog (1962).