Comparison of steam pretreatment of eucalyptus, aspen, and spruce wood chips and their enzymatic hydrolysis
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- Ramos, L.P., Breuil, C. & Saddler, J.N. Appl Biochem Biotechnol (1992) 34: 37. doi:10.1007/BF02920532
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Enzymatic hydrolysis of SO2-impregnated, steam-explodedEucalyptus viminalis was carried out at increasing substrate concentrations and enzyme loadings. When low enzyme loadings were used, the peroxide-treated fraction derived from eucalyptus chips (SEE-WIA/ H2O2) was more readily hydrolyzed than the water-insoluble (SEE-WI) and the alkali-insoluble (SEE-WIA) fractions. The various cellulosic fractions derived from steam-explodedE. viminalis exhibited a greater susceptibility to hydrolysis than the equivalent aspen and spruce substrates, particularly at high substrate concentrations (10%, w/v).